TNNT1在晚期非小细胞肺癌中的表达及与患者预后的关系  

The Expression of TNNT1 in Advanced Non-small Cell Lung Cancer and its Relationship with the Prognosis

作　　者：邓浩 朱洪斌[1] 朱楠[1] 王瑞[1] DENG Hao;ZHU Hong-bin;ZHU Nan;WANG Rui(Department of Respiratory and Critical Care Medicine,Chaohu Hospital of Anhui Medical University,Chaohu 238000,Anhui,China)

机构地区：[1]安徽医科大学附属巢湖医院呼吸与危重症医学科,安徽巢湖238000

出　　处：《医学信息》2023年第5期102-107,共6页Journal of Medical Information

基　　金：2021年安徽高校自然科学研究重点项目(编号:KJ2021A0336)。

摘　　要：目的探讨肌钙蛋白T1(TNNT1)在晚期非小细胞肺癌中的表达情况及其临床价值。方法首先使用Ualcan在线分析网站对肿瘤基因组计划(TCGA)中非小细胞肺癌基因表达谱数据和临床数据进行挖掘,探讨TNNT1 mRNA在非小细胞肺癌中的表达情况,分析其与预后的关系;进一步选取安徽医科大学附属巢湖医院2018年1月-2019年9月收治并经病理确诊的晚期非小细胞肺癌80例,采用免疫组织化学染色方法(IHC)检测患者病理样本及正常肺组织中TNNT1蛋白的表达,分析其与临床病理特征及预后的关系。结果TCGA数据库分析显示,非小细胞肺癌组织中TNNT1 mRNA表达较癌旁正常组织高(P<0.05),肺腺癌中TNNT1 mRNA高表达的总生存时间较TNNT1 mRNA中低表达的患者短(P<0.05);IHC检测显示,TNNT1蛋白在晚期非小细胞肺癌组织中呈高表达,在正常肺组织中呈低表达;TNNT1蛋白的表达水平与淋巴结转移以及TNM分期有关(P<0.05);Kaplan-Meier生存分析显示,TNNT1高表达患者的预后不良;Cox回归分析显示,TNNT1的表达水平和TNM分期是影响患者总生存期(OS)及无进展生存期(PFS)的独立危险因素。结论TNNT1蛋白在晚期非小细胞肺癌中呈异常高表达,并与患者不良预后相关,是影响患者预后的独立危险因素。Objective To investigate the expression and clinical value of troponin T1(TNNT1)in advanced non-small cell lung cancer.Methods Firstly,the Ualcan online analysis website was used to mine the gene expression profile data and clinical data of non-small cell lung cancer in the Cancer Genome Atlas(TCGA)to explore the expression of TNNT1 mRNA in non-small cell lung cancer and analyze its relationship with prognosis.Further,80 patients with advanced non-small cell lung cancer admitted to Chaohu Hospital of Anhui Medical University from January 2018 to September 2019 were selected.Immunohistochemical staining(IHC)was used to detect the expression of TNNT1 protein in pathological samples and normal lung tissues,and its relationship with clinicopathological features and prognosis was analyzed.Results TCGA database analysis showed that the expression of TNNT1 mRNA in non-small cell lung cancer tissues was higher than that in adjacent normal tissues(P<0.05).The overall survival time of patients with high expression of TNNT1 mRNA in lung adenocarcinoma was shorter than that of patients with low expression of TNNT1 mRNA(P<0.05).IHC detection showed that TNNT1 protein was highly expressed in advanced non-small cell lung cancer tissues and lowly expressed in normal lung tissues,the expression level of TNNT1 protein was related to lymph node metastasis and TNM stage(P<0.05).Kaplan-Meier survival analysis showed that patients with high expression of TNNT1 had poor prognosis.Cox regression analysis showed that TNNT1 expression and TNM stage were independent risk factors for overall survival(OS)and progression-free survival(PFS).Conclusion TNNT1 protein is abnormally high expressed in advanced non-small cell lung cancer,which is associated with poor prognosis and is an independent risk factor affecting the prognosis of patients.

关 键 词：肌钙蛋白T1 非小细胞肺癌 总生存期 淋巴结转移 

分 类 号：R734.2[医药卫生—肿瘤]