8周负重爬梯抗阻训练对小鼠右心室心肌特定miRNAs的影响  

Effects of 8-week Loading Ladder Resistance Training on Specific miRNAs in Right Ventricular Myocardium of Mice

作　　者：宋彦霖 张海鹏 徐琳 白荣雁 徐盛嘉 马继政 SONG Yanlin;ZHANG Haipeng;XU Lin;BAI Rongyan;XU Shengjia;MA Jizheng(School of Exercise Health,Nanjing Sport Institute,Nanjing Jiangsu,210014;Jiangsu Provincial Judicial Police Officer Higher Vocational and Technical School,Zhenjiang Jiangsu,212000;The Research Center of Military Exercise Science,The Army Engineering University of PLA,Nanjing Jiangsu,211101)

机构地区：[1]南京体育学院运动健康学院,江苏南京210014 [2]江苏司法警官高等职业学校,江苏镇江212000 [3]陆军工程大学军事运动科学研究中心,江苏南京211101

出　　处：《湖北体育科技》2023年第2期106-111,共6页Hubei Sports Science

基　　金：2021年基础加强计划技术领域基金项目(2021-JCJQ-JJ-1021);2021年江苏省自然科学基金(Bk20211228);2021年江苏省普通高校学术学位研究生科技创新计划项目(KYCX20_1983);2021年陆军工程大学基础前沿科技创新基础研究类项目。

摘　　要：目的 在剧烈运动期间,右心室会承受更多的后负荷和室壁应力,本研究评定8周抗阻训练对小鼠右心室心肌特定mi RNAs的表达产生的影响,是否参与调控右心室心肌肥大。方法 12只C57BL/6J雄性小鼠,依据体重随机分为对照组(CON组)和抗阻训练组(LRT组),每组6只。两组在预适应、抓力测试和最大自愿承载力测试后,LRT组进行8周的尾部负重爬梯训练,CON组正常饲养不进行爬梯训练。记录两组小鼠1到9周的体重、第9周的体脂率以及第1、5、9周的抓力和最大自愿承载力测试成绩,在所有测试结束48h后,取小鼠右心室心肌,利用实时荧光定量PCR检测心肌肌组织特异的mi RNAs及其他mi RNAs的表达变化情况。使用免疫印迹检测PI3K、Akt和m TOR蛋白表达量变化情况。结果 训练8周后,LRT组小鼠心脏湿重显著升高(p<0.01);抓力和最大自愿承载力测试成绩显著上升(p<0.05;p<0.01);与CON组相比,LRT组右心室mi R-208a、mi R-21、mi R-222、mi R-486表达显著上升(p<0.01;p<0.01;p<0.05;p<0.001),分别升高了0.9倍、0.8倍、0.5倍和0.7倍。其中,mi R-208a和mi R-222与心脏湿重呈正相关(r=0.8391,p<0.05;r=0.8147,p<0.05)。mi R-208b、mi R-499、mi R-1、mi R-17-3p、mi R-133a和mi R-133b在抗阻训练后的右心室心肌中没有显著变化(p>0.05)。抗阻训练不影响这些mi RNAs的表达。LRT组小鼠右心室心肌中PI3K、Akt和m TOR蛋白表达量显著升高(p<0.01;p<0.05;p<0.05)。结论8周尾部负重抗阻训练能够诱导小鼠心脏右心室肥大且激活PI3K/Akt/mTOR信号通路;mi R-208a和mi R-222可能参与调控抗阻运动引起的心脏右心室肥大。Objective During strenuous exercise,the right ventricle will bear more afterload and wall stress.It evaluated the effect of 8 weeks of resistance training on the expression of specific miRNAs in the right ventricular myocardium of mice,and whether it was involved in the regulation of right ventricular myocardial hypertrophy.Methods Twelve male C57BL/6J mice were randomly divided into control group(CON group)and resistance training group(LRT group)according to body weight,with 6 mice in each group.After pre-acclimation,grip test and maximum voluntary carrying capacity test,LRT group was trained with tail load climbing for 8 weeks,while CON group was fed normally without ladder climbing exercise.The body weight of mice from 1 to 9 weeks,the body fat percentage of mice at 9 weeks,and the grip and maximum voluntary carrying capacity of mice at 1,5 and 9 weeks were recorded.After 48 hours of all tests,the right ventricular myocardium of mice was taken,and the expression changes of myocardial tissue specific miRNAs and other miRNAs were detected by real-time PCR.Western blotting was used to detect the expression of PI3K,Akt and mTOR proteins.Results After 8 weeks of training,the wet heart weight of mice in LRT group was significantly increased(p<0.01).The test scores of grip and maximum voluntary carrying capacity were significantly increased(p<0.05;p<0.01).Compared with the CON group,the expressions of miR-208a,miR-21,miR-222 and miR-486 in the right ventricle of the LRT group were significantly increased(p<0.01;p<0.01;p<0.05;p<0.001),increased 0.9 times,0.8 times,0.5 times and 0.7 times,respectively,miR-208a and miR-222 were positively correlated with heart wet weight(r=0.8391,p<0.05;r=0.8147,p<0.05),miR-208b,miR-499,miR-1,miR-17-3p,miR-133a and miR-133b had no significant changes in right ventricular myocardium after resistance training(p>0.05).Resistance training did not affect the expression of these miRNAs.The protein expressions of PI3K,Akt and mTOR in right ventricular myocardium of mice in LRT group were signi

关 键 词：mi RNAs 右心室心肌 抗阻训练 小鼠实验 

分 类 号：G804.7[文化科学—运动人体科学]