骨甘蛋白在胃癌细胞干性维持及对化疗敏感性中的作用  

The role of osteoglobin in the maintenance of gastric cancer cell stemness and sensitivity to chemotherapy

作　　者：邹岩[1] 姜桂春[1] 李辉[2] 谢艳敏[1] Zou Yan;Jiang Guichun;Li Hui;Xie Yanmin(Endoscopy Center,Liaoning Province Cancer Hospital&Institute,Shenyang 110042,Liaoning,China;Nursing Department,Liaoning Province Cancer Hospital&Institute,Shenyang 110042,Liaoning,China)

机构地区：[1]辽宁省肿瘤医院内镜中心,辽宁沈阳110042 [2]辽宁省肿瘤医院护理部,辽宁沈阳110042

出　　处：《消化肿瘤杂志（电子版）》2022年第4期431-436,共6页Journal of Digestive Oncology(Electronic Version)

基　　金：医工交叉联合基金(LD202118)。

摘　　要：目的探究骨甘蛋白(OGN)对胃癌(GC)细胞干性与化疗耐药性的影响。方法实时荧光聚合酶链反应(RT-PCR)、蛋白质印迹法、免疫组织化学(IHC)联合癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库分析OGN在胃癌细胞和组织中的表达。CCK-8和克隆形成评估细胞增殖能力变化,通过流式细胞术对细胞凋亡进行测定,CCK-8检测OGN在GC细胞化疗抵抗中的作用。蛋白质印迹法检测细胞干性相关分子标志物表达变化。结果OGN在GC细胞表达低于其在胃黏膜细胞中表达(P<0.05);过表达OGN的GC细胞侵袭能力减弱(P<0.05),凋亡增加(P<0.05)。在铂类和氟尿嘧啶处理后,细胞存活比例下降,化疗抵抗减轻(P<0.05),OGN诱导了化疗耐药。过表达OGN抑制了干性标志物的表达。结论OGN在GC组织中低表达,过表达的OGN能够抑制细胞干性,影响化疗耐药。Objective To investigate the effect of osteoglycin(OGN)on the stemness and chemotherapy resistance of gastric cancer(GC)cells.Method Real-time polymerase chain reaction(RT-PCR),westernblot,immunohistochemistry(IHC)combined with The Cancer Genome Atlas(TCGA)database to analyze OGN in GC cells and tissues expression.CCK-8 and clone formation assaywere used to assess changes in cell proliferation ability,and apoptosis was measured by flow cytometry.CCK-8 was used to detect the role of OGN in chemoresistance of GC cells.Western-blot detection of cell stemness-related molecular markers expression changes.Result The expression of OGN in GC cells was lower than that in gastric mucosal cells(P<0.05).Overexpressed OGN decreased the invasive ability of GC cells(P<0.05)and increased apoptosis(P<0.05).After platinum and fluorouracil treatment,the survival rate of cells decreased and chemotherapy resistance was alleviated(P<0.05),and OGN induced chemotherapy resistance.Overexpression of OGN suppressed the expression of stemness markers.Conclusion OGN is weakexpressed in GC tissues,and overexpressed OGN can inhibit cell stemness and affect chemotherapy resistance.

关 键 词：胃癌 骨甘蛋白 增殖 凋亡 干性 化疗耐药 

分 类 号：R735.2[医药卫生—肿瘤]