芳香万寿菊叶片自然释放的挥发性有机物构成  

Phytochemical Analysis of Volatile Organic Compounds from Tagetes lemmonii

作　　者：刘畅[1] 郭阿君[1] Liu Chang;Guo Ajun(Beihua University,Jilin 132013,P.R.China)

机构地区：[1]北华大学,吉林省吉林市132013

出　　处：《东北林业大学学报》2023年第3期67-72,共6页Journal of Northeast Forestry University

摘　　要：明确芳香万寿菊(Tagetes lemmonii)的挥发性有机物(VOCs)成分,是对其芳香特性深度应用的理论基础。为了分析不同时段芳香万寿菊新鲜叶片及干燥叶片自然释放的挥发性有机物,利用固相微萃取-气质联用技术(SPME-GC-MS)对其进行分析比较。结果表明:生长阶段的芳香万寿菊鉴定出10类共57种挥发性有机化合物,5月20日—25日、7月20—25日共同存在的挥发性有机物11种,均以酮类、萜类、醇类化合物为主,酮类质量分数最大,醇类质量分数最小。酮类化合物以(Z)-2,6-二甲基-2,5,7-三烯-4-酮、(Z)-2,6-二甲基-5,7-二烯-4-酮、2,6-二甲基辛-7-烯-4-酮为主;萜类化合物以(+)-柠檬烯、(E)-Β-罗勒烯、桧烯为主;醇类化合物以桉叶油醇、芳樟醇为主。干燥叶片鉴定出12类共53种挥发性有机化合物,不同存放时间的干叶共同存在的挥发性有机物7种;存放3个月后,新增蒎烯、3-甲基-2-(3-甲基-2-丁烯基)呋喃、(E)-2,6-二甲基-5,7-二烯-4-酮、(Z)-2,6-二甲基-2,5,7-三烯-4-酮等化合物34种。因此芳香万寿菊不同时段叶片及自然干燥叶片挥发性有机物成分及释放量差异显著。Volatile components from Tagetes lemmonii in the fresh leaf and dried leaves were determined by solid-phase microextraction(SPME) coupled with gas chromatography-mass spectrometry(GC-MS). In spring, summer and fall, a total of 57 compounds were identified from 10 classes of VOCs, and Ketones, terpenoids and alcohols were the major constituents. The results showed that: relative amount from high to low was ketones, terpenoids and alcohols. There are 11 identical substances from fresh leaf VOCs in spring, summer and fall. The main substances were(Z)-Tagetenone, 5,7-Octadien-4-one, 2,6-dimethyl-,(Z)-, 7-Octen-4-one, 2,6-dimethyl-from ketones. D-Limonene, trans-.beta.-Ocimene and sabinenewere the main substances from terpenoids. The main compound are Eucalyptol and Linalool from alcohols. A total of 53 compounds were identified from 12 classes of VOCs from dried leaves stored for 1 and 3 months. The results shown that D-Limonene, 5,7-Octadien-4-one, 2,6-dimethyl-,(Z)-, 7-Octen-4-one, 2,6-dimethyl-were the main substances.There are 7 identical substances in VOCs from dried leaves stored for 1 month and 3 months. After 3 months, 34 substances were added to the volatiles from dried leaves stored for 3 months, including(+)-α-pinene, α-Naginatene,(E)-Tagetone and(Z)-Tagetenone. This study clarified the differences of VOCs composition and relative amount from leaves in different seasons and storage time of Tagetes lemmonii.

关 键 词：芳香万寿菊 固相微萃取 挥发性有机物 

分 类 号：S715.3[农业科学—林学]