CD68和CD166在结直肠癌组织中的表达及临床意义  

Expression and clinical significance of CD68 and CD166 in colorectal cancer tissues

作　　者：武会斌 王皇建 王云帅 夏添明 李朝辉 韩保卫 WU Huibin;WANG Huangjian;WANG Yunshuai(Department of General Surgery,The Second Affiliated Hospital of Zhengzhou University,Henan,Zhengzhou 450053,China;不详)

机构地区：[1]郑州大学第二附属医院普外科,郑州市450053 [2]郑州大学附属洛阳中心医院胃肠外科

出　　处：《河北医药》2023年第3期348-352,共5页Hebei Medical Journal

基　　金：河南省科技攻关计划项目(编号:1921023100395);河南省医学科技攻关计划联合共建项目(编号:2018020898,LHGJ20191223)。

摘　　要：目的研究结直肠癌(CRC)组织中肿瘤相关巨噬细胞(TAMs)标志物CD68和肿瘤干细胞(CSCs)标志物CD166表达变化,探讨其与CRC患者临床病理特征及预后的关系。方法选取2014年1月至2019年10月CRC患者148例,取术中切除癌组织和配对的癌旁正常组织(距癌组织边缘>5 cm),应用免疫组化SABC法检测CD68^(+)TAMs与CD166^(+)CSCs的表达。比较癌组织与癌旁正常组织CD68^(+)TAMs、CD166^(+)CSCs阳性表达率;比较不同临床病理特征CRC患者癌组织CD68^(+)TAMs、CD166^(+)CSCs表达水平,Spearman相关法分析癌组织CD68^(+)TAMs、CD166^(+)CSCs表达的相关性;Kaplan-Meier法及Log-Rank检验比较CD68^(+)TAMs与CD166^(+)CSCs无高表达组、单一高表达组及均高表达组的5年生存率;多因素Cox回归分析CRC患者预后不良的影响因素。结果CRC患者癌组织CD68^(+)TAMs、CD166^(+)CSCs阳性表达率均高于癌旁正常组织(P<0.05)。CD68^(+)TAMs、CD166^(+)CSCs表达水平与肿瘤大小、淋巴结转移、TNM分期及分化程度密切相关,差异均有统计学意义(P<0.05),且二者的表达水平呈正相关(r=0.777,P<0.01)。CD68^(+)TAMs、CD166^(+)CSCs无高表达组、单一高表达组、均高表达组的5年生存率依次降低,差异有统计学意义(P<0.05)。肿瘤大小、淋巴结转移、TNM分期、CD68^(+)TAMs和CD166^(+)CSCs联合分组与患者的总生存期有关(P<0.05),且淋巴结转移、TNM分期、CD68^(+)TAMs和CD166^(+)CSCs联合分组为影响患者总生存期的独立因素(P<0.05)。结论CD68^(+)TAMs与CD166^(+)CSCs在CRC的发生发展中可能起协同促进作用,联合检测二者可进一步提高对CRC患者预后的评估价值,并指导临床进行更为精准的治疗。Objective To study changes in the expressions of tumor-associated macrophages(TAMs)marker CD68 and cancer stem cell(CSCs)marker CD166 in colorectal cancer(CRC)tissues,and how to impact on clinicopathological features and prognosis of CRC patients.Methods A total of 148 CRC patients from January 2014 to October 2019 in the second affiliaoted hospital of Zhengzhou University were enrolled as subjects,intraoperative resected cancer tissues and paired adjacent normal tissues(distance from the edge of cancer tissue>5cm)were collected.The expressions of CD68^(+)TAMs and CD166^(+)CSCs were immunostained by SABC method,aimging to compare the positive expression rate and clinicopathological characteristics of CD68^(+)TAMs and CD166^(+)CSCs in cancer tissues and adjacent normal tissues in cancer tissues of CRC patients.Spearman correlation method was performed to analyze the correlation between CD68^(+)TAMs and CD166^(+)CSCs in cancer tissues.Kaplan Meier method and Log rank test were conducted to assess the 5-year survival rates in no-high expression group,single-high expression group and the combined high expression group.Multivariate Cox regression was applied to analyze the factors affecting poor prognosis.Results The positive expression rate of CD68^(+)TAMs and CD166^(+)CSCs in cancer tissues of CRC patients was higher than that of adjacent normal tissues(P<0.05),and the differences were statistically significant(P<0.001).The expression level of CD68^(+)TAMs and CD166^(+)CSCs was closely related to the tumor size,lymph node metastasis,and TNM’s stage,and the difference was statistically significant(P<0.05),and the expression level of two indexes were significantly positively correlated(r=0.777,P<0.01).The 5-year survival rate of CD68^(+)TAMs and CD166^(+)CSCs in no-high expression group,single-high expression groups,and the combined high expression was lowered gradually,and the differences were statistically significant(P<0.05).Tumor size,lymph node metastasis and TNM’s stage,and pooled CD68^(+)TAMs and CD166^(+)C

关 键 词：结直肠癌 CD68 CD166 临床病理特征 预后 

分 类 号：R735.35[医药卫生—肿瘤]