NSCLC患者癌组织Napsin A、TTF-1、ALK、Ki-67表达与临床病理的关系及免疫组化检测意义  

The Relationship Between the Expressions of Napsin A,TTF-1,ALK,Ki-67 and Clinicopathological Characteristics in NSCLC Patients and the Significance of Immunohistochemistry

作　　者：罗教秀 孙世珺[1] 孟凤娇 林绮璐 LUO Jiaoxiu;SUN Shijun;MENG Fengjiao;LIN Qilu(Department of Pathology,Zhongshan People’s Hospital,Zhongshan 528403,China)

机构地区：[1]中山市人民医院病理科,广东中山528403

出　　处：《标记免疫分析与临床》2022年第12期2064-2070,共7页Labeled Immunoassays and Clinical Medicine

基　　金：2021年度中山市第一批社会公益与基础研究项目(编号:2021B1033)。

摘　　要：目的分析非小细胞肺癌(NSCLC)患者癌组织新天门冬氨酸蛋白酶A(Napsin A)、甲状腺转录因子-1(TTF-1)、间变性淋巴瘤激酶(ALK)、Ki-67抗原(Ki-67)表达与临床病理的关系及免疫组化检测意义。方法回顾性收集2020年5月至2022年5月中山市人民医院收治的62例NSCLC患者临床资料,所有患者均进行手术治疗,收集患者手术切除标本进行免疫组化病理检查。统计NSCLC患者癌组织和癌旁组织中Napsin A、TTF-1、ALK、Ki-67阳性表达情况及不同病理特征,NSCLC患者癌组织Napsin A、TTF-1、ALK、Ki-67阳性表达情况,采用Spearman相关法分析NSCLC患者癌组织Napsin A、TTF-1、ALK、Ki-67表达的相关性。结果NSCLC患者癌组织中Napsin A、TTF-1、ALK、Ki-67阳性表达率(79.03%、70.97%、17.74%、82.26%)高于癌旁组织(16.13%、22.58%、0.00%、0.00%,P<0.05)。鳞癌、高/中分化、无淋巴结转移、无远处转移NSCLC患者癌组织Napsin A阳性表达率低于腺癌、低分化、淋巴结转移、远处转移患者(P<0.05);低分化、临床分期Ⅲ/Ⅳ期、淋巴结转移、远处转移NSCLC患者癌组织TTF-1阳性表达率高于高/中分化、临床分期Ⅰ/Ⅱ期、无淋巴结转移、无远处转移患者(P<0.05);鳞癌、低分化、临床分期Ⅲ/Ⅳ期NSCLC患者癌组织ALK阳性表达率高于腺癌、高/中分化、临床分期Ⅰ/Ⅱ期患者(P<0.05);低分化、临床分期Ⅲ/Ⅳ期、淋巴结转移、远处转移NSCLC患者癌组织Ki-67表达率高于高分化、临床分期Ⅰ/Ⅱ期、无淋巴结转移、无远处转移患者(P<0.05)。Spearman相关法分析结果显示,NSCLC患者癌组织Napsin A表达与ALK表达呈显著正相关关系(r=0.589,P<0.05),TTF-1表达与ALK、Ki-67表达呈显著正相关关系(r=0.564、0.602,P<0.05)。结论NSCLC的发生及其病情进展可引起Napsin A、TTF-1、ALK、Ki-67表达升高,患者癌组织中Napsin A、ALK及TTF-1、ALK、Ki-67表达具有一定相关性,其可能在NSCLC的发生和发展中相互作用。Objective To analyze the relationship between the expressions of new aspartate proteinase A(Napsin A),thyroid transcription factor-1(TTF-1),anaplastic lymphoma kinase(ALK),Ki-67 antigen(Ki-67)and clinicopathological characteristics in non-small cell lung cancer(NSCLC)and the significance of immunohistochemical detection.Methods The clinical data of 62 patients with NSCLC admitted to Zhongshan People’s Hospital from May,2020 to May,2022 were retrospectively collected.All patients underwent surgical treatment,and the resected specimens were collected for immunohistochemical pathological examination.The positive expressions of Napsin A,TTF-1,ALK and Ki-67 in cancer tissues and adjacent tissues of NSCLC patients and the positive expressions of Napsin A,TTF-1,ALK and Ki-67 in cancer tissues of NSCLC patients with different pathological characteristics were analyzed.Spearman correlation was used to analyze the correlation between the expressions of Napsin A,TTF-1,ALK and Ki-67 in NSCLC patients.Results The positive expression rates of Napsin A,TTF-1,ALK and Ki-67 in cancer tissues of NSCLC patients(79.03%,70.97%,17.74%,82.26%)were higher than those in adjacent tissues(16.13%,22.58%,0.00%,0.00%)(P<0.05).The positive expression rates of Napsin A in squamous cell carcinoma,high/medium differentiation,no lymph node metastasis and no distant metastasis were lower than that in adenocarcinoma,low differentiation,lymph node metastasis and distant metastasis(P<0.05).The positive expression rates of TTF-1 in cancer tissues of NSCLC patients with poorly differentiated NSCLC,clinical stage Ⅲ/Ⅳ,lymph node metastasis and distant metastasis were higher than that of patients with high/moderate differentiation,clinical stage Ⅰ/Ⅱ,no lymph node metastasis and no distant metastasis(P<0.05).The positive expression rates of ALK in patients with squamous cell carcinoma,poorly differentiated NSCLC and clinical stage Ⅲ/Ⅳ were higher than that in patients with adenocarcinoma,high/moderate differentiated NSCLC and clinical stage �

关 键 词：非小细胞肺癌 癌组织 新天门冬氨酸蛋白酶A 甲状腺转录因子-1 间变性淋巴瘤激酶 Ki-67抗原 临床病理 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]