利用InDel标记基于玉米胚乳基因型的亲子鉴定方法  

Parentage Identification Based on Maize Endosperm Genotypes Using InDel Markers

作　　者：刘志浩[1,2] 赵怡锟 梁紫越 许理文 王凤格[2] 路运才[1] LIU Zhi-hao;ZHAO Yi-kun;LIANG Zi-yue;XU Li-wen;WANG Feng-ge;LU Yun-cai(School of Modern Agriculture and Ecology,Heilongjiang University,Harbin 150080;Maize Research Center/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097;School of Agriculture,China Agricultural University,Beijing 100193,China)

机构地区：[1]黑龙江大学现代农业与生态环境学院,哈尔滨150080 [2]北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室,北京100097 [3]中国农业大学农学院,北京100193

出　　处：《玉米科学》2023年第1期48-53,共6页Journal of Maize Sciences

基　　金：国家十三五重点研发计划项目“玉米分子设计育种”(2016YFD0101803)。

摘　　要：基于玉米胚乳中母本和父本DNA的2∶1的剂量关系,利用20个InDel标记在荧光毛细管电泳平台上对玉米进行亲子鉴定并得到玉米杂交种三联体的指纹数据。研究利用8个玉米杂交种单株的胚及胚乳DNA得到在11个杂和位点上每个基因型的峰面积,并计算得出胚和胚乳各自的等位基因扩增比(左峰面积/右峰面积)。通过比较发现,胚及胚乳之间的等位基因扩增比具有显著差异。在其中4个标记上(IDP054、IDP151、IDP161、IDP238),胚乳DNA等位基因扩增比较胚DNA低,胚乳的右峰面积较大,说明右峰代表基因型的DNA含量较高,判断该位点右峰代表的基因型为母本基因型。依此判断另外7个标记的左峰代表基因型为母本基因型,最终得到玉米杂交种三联体的指纹数据,通过与杂交种双亲的指纹进行比对验证判断结果的正确性。Twelve InDel markers were used on a fluorescent capillary electrophoresis platform to identify the parentage of maize based on the 2∶1 dosage relationship between maternal and paternal DNA in maize endosperm and obtained the fingerprint data of maize hybrid triplets.The peak areas of each genotype at 11 heterozygous loci were obtained using embryo and endosperm DNA from 8 maize hybrids and the amplification ratio(left peak area/right peak area)was calculated for each allele of embryo and endosperm.The allele amplification ratios between em⁃bryo and endosperm were significantly different,and on 4 of the markers(IDP054,IDP151,IDP161,IDP238),the endosperm DNA allele amplification ratio was lower than that of the embryo DNA,and the right peak area of the en⁃dosperm was larger,indicating that the DNA content of the genotype represented by the right peak was higher,and the genotype represented by the right peak at this locus was judged to be the maternal genotype;accordingly,the left peak of the other 7 markers was judged to be the maternal genotype,and the fingerprint data of the maize hybrids triplet were finally obtained;and the correctness of the judgment results was verified by comparing with the finger⁃prints of both parents of the hybrids.

关 键 词：玉米 胚乳 INDEL 荧光毛细管电泳 亲子鉴定 

分 类 号：S513.035.2[农业科学—作物学]