P_(luxI)mutants with different promoting period and their application for quorum sensing regulated protein expression  

作　　者：Zhuoning Cao Zhen Liu Guilin Zhang Xiangzhao Mao 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China

出　　处：《Food Science and Human Wellness》2023年第5期1841-1849,共9页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(31922072);Natural Science Foundation of Shandong Province(ZR2020JQ15);Project of Shandong Province Higher Educational Science and Technology Program(2019KJF012);Taishan Scholar Project of Shandong Province(tsqn201812020);China Agriculture Research System(CARS-48);Qingdao Science and Technology Demonstration and Guidance Project for Benefiting the People(20-3-4-28-nsh)。

摘　　要：Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia coli.To enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)mutants.To test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for expression.The enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 promoter.In high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 promoter.Therefore,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.

关 键 词：Quorum sensing Promoter engineering ENZYMES Alginate lyase ESTERASE High-density fermentation 

分 类 号：TS2[轻工技术与工程—食品科学与工程]