A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity,and antitumor activity in a B16 melanoma mouse model  

作　　者：Yuanhui Li Peng Wang Zejun Zhang Qinghong Liu 

机构地区：[1]Department of Vegetables,College of Horticulture,China Agricultural University,Beijing 100193,China [2]College of Food Science&Nutritional Engineering,China Agricultural University,Beijing 100193,China

出　　处：《Food Science and Human Wellness》2023年第5期1885-1892,共8页食品科学与人类健康（英文）

基　　金：supported by grants from the China Agriculture Research System(CARS-20-01A)。

摘　　要：A novel lectin(termed PML)was purified from fruiting bodies of the edible mushroom Phellodon melaleucus(division Basidiomycota)by ion exchange,hydrophobic interaction,and gel filtration chromatographies,with overall titer recovery~60%and 20-fold purification.PML displayed hemagglutination activity 13319 units/mg toward rabbit erythrocytes.SDS-PAGE and gel filtration analyses revealed that PML is a homodimeric lectin with a molecular weight of 28.8 kDa.PML hemagglutination activity was not inhibited by various simple sugars or their derivatives,but was enhanced by cations Ca^(2+),Mg^(2+),Zn^(2+),and Cu^(2+).The activity was stable in pH range 6–9 and in the temperature range 20–60°C.Circular dichroism(CD)spectroscopic analysis showed that PML was composed primarily ofβ-sheets with lowα-helix content.In a B16 melanoma mouse model,PML treatment significantly inhibited tumor growth,and increased cytokine IL-10 content.Our findings suggest that PML is a potential anticancer therapeutic agent.

关 键 词：Phellodon melaleucus LECTIN Hemagglutination activity Cytokine IL-10 Antitumor activity 

分 类 号：R285.5[医药卫生—中药学]