穿刺小标本改良石蜡块制作方法及评价  

An improved paraffin embedding method for small core needle biopsy: technical introduction and evaluation

作　　者：陈宛灵 石超吉[1] 王敏[1] 顾挺[1] 田臻[1] CHEN Wan-ling;SHI Chao-ji;WANG Min;GU Ting;TIAN Zhen(Department of Oral Pathology,Shanghai Ninth Peo-ple's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stoma-tology,Shanghai Research Institute of Stomatology.Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔病理科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011

出　　处：《上海口腔医学》2023年第1期6-11,共6页Shanghai Journal of Stomatology

基　　金：上海市自然科学基金(22ZR1437000)。

摘　　要：目的 :改良现有琼脂预包埋石蜡包埋法,评价该包埋法对空芯针穿刺活检小标本组织完整性、组织学形态、蛋白质和DNA检测的影响。方法:取10例口腔黏膜鳞癌空芯针穿刺活检标本,分别进行成型包埋模具的改良琼脂预包埋石蜡包埋法及常规石蜡包埋法,前者缩短脱水时间至3.5 h,后者为12 h。样本处理后,分别行H-E染色、组织学形态、免疫组织化学(IHC)和DNA荧光原位杂交(FISH)等检测,并对检测结果进行对比分析。采用GraphPad Prism 9软件包对数据进行组间t检验。结果:改良琼脂预包埋法较琼脂预包埋法降低了操作难度,易于推广。与常规石蜡包埋法相比,组织脱水时间显著缩短(P<0.001),对镜下组织学形态及后续IHC和FISH检测无影响。结论:改良琼脂预包埋石蜡包埋法满足临床病理诊断对组织处理的要求,是值得推广的空芯针穿刺活检标本组织包埋法。PURPOSE: Modified agar pre-embedded paraffin embedding method was proposed to evaluate the effects on tissue integrity, histological morphology, protein and DNA detection in small specimens of core needle biopsies. METHODS:The core needle biopsy specimens of 10 patients with oral mucosal squamous cell carcinoma were subjected to modified agar pre-embedded paraffin embedding using molded embedding molds and conventional paraffin embedding respectively,the dehydration time of the former was 3.5 h and that of the latter was 12 h. After tissue treatment, H-E staining, histo-logical morphology, immunohistochemistry(IHC), and DNA fluorescence in situ hybridization(FISH) were performed, respectively. The results were compared and analyzed using GraphPad Prism 9 software package. RESULTS: The modified agar pre-embedding method was less difficult to perform than the agar pre-embedding method, and easier to be promoted.Compared with conventional paraffin embedding method, the tissue dehydration time was significantly reduced(P<0.001),and the results of microscopic histological morphology and subsequent IHC and FISH assays were reliable. CONCLUSIONS: The modified agar pre-embedded paraffin embedding method meets the requirements of clinical pathological diagnosis for tissue processing, and is worthy of clinical application for core needle biopsy specimens.

关 键 词：空芯针穿刺活检 琼脂预包埋 石蜡包埋 

分 类 号：R780.2[医药卫生—口腔医学]