2019年福建省漳州市本地流行登革病毒的E基因序列分析  

Senquence analysis of envelope gene of local dengue virus in Zhangzhou city, Fujian province, 2019

作　　者：郭丽清[1] 许美燕[1] 陈秋虾 杨彬彬[1] 王金章[2] Guo Liqing;Xu Meiyan;Chen Qiuxia;Yang Binbin;Wang Jinzhang(Zhangzhou Center for Disease Control and Prevention,Zhangzhou 363005,China;Fujian Provincial Center for Disease Control and Prevention,Fujian Provincial Key Laboratory of Zoonosis Research,Fuzhou 350012,China)

机构地区：[1]漳州市疾病预防控制中心,漳州363005 [2]福建省疾病预防控制中心、福建省人兽共患病研究重点实验室,福州350012

出　　处：《中华实验和临床病毒学杂志》2023年第1期50-54,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解2019年漳州市本地登革热疫情病原学特征及可能的传染来源。方法采集疑似登革热患者急性期的血清样本, 通过实时荧光定量RT-PCR进行病毒检测和血清分型。对于阳性标本, 通过RT-PCR扩增E基因的全长片段, 并进行测序和分析。结果 2019年漳州市共有登革热本地病例98例, 集中在诏安县、龙海区、云霄县3个区县。本研究中选取了代表各区县不同来源的14株登革病毒E基因序列, 其氨基酸序列毒力位点分析发现本地流行株均属于毒性相对较强的毒株, 系统进化树显示本地流行株均为DENV-I, 其基因型为I型, 分为a、b、c进化分支。a进化分支包含了所有诏安县和龙海区序列, 又分成了3个亚分支, b、c进化分支均为云霄县的序列, 除诏安县深桥镇和龙海区海澄镇的毒株关联度较高, 其余地区均局限于各乡镇所在范围, 且各进化分支均与同年流行的国内其他地区及东南亚相应国家较为接近。结论 2019年漳州市登革热本地疫情暴发存在多个输入来源, 可能是由国内其他地区或者东南亚国家的登革热输入病例引起的, 同时存在本地的跨县域传播可能。Objective To analyze the epidemiological characteristics and track probable imported sources of the local dengue outbreak in Zhangzhou city,Fujian province,2019.Methods Serum samples of patients with suspected dengue fever at acute phases were collected for virus detecting and serotyping by real-time fluorescence quantitative RT-PCR.For the positive specimens of local cases,full-length fragments of E gene were amplified by RT-PCR,and were sequenced and analyzed.Result In 2019,there were 98 local cases of dengue fever in Zhangzhou city,which were concentrated in Zhao’an county,Longhai district and Yunxiao county.In this study,fourteen dengue virus E gene sequences representing different sources in different districts and counties were selected.The amino acid sequence virulence site analysis showed that the local epidemic strains were relatively virulent strains.The gene sequence alignment and phylogenetic analysis showed that all the local strains were classified as DENV-I subgenotype genotype I,divided into a,b and c three different branches.The evolutionary branch a contained all Zhao’an and Longhai sequences and was divided into three sub-branches,the b and c evolutionary branches were both the sequences of Yunxiao.There was a high correlation between the Shenqiao Town in Zhao’an and the Haicheng Town in Longhai.The other areas of the strains were limited to the towns,and the evolutionary branches were close to the other areas in China and countries in Southeast Asia.Conclusions The indigenous dengue outbreaks in Zhangzhou,2019 were caused by multiple sources of introduction and originated from other areas in China or from Southeast Asian countries and there was also the possibility of local cross-county transmission.

关 键 词：登革热 登革病毒 E基因 系统进化树 

分 类 号：R373.33[医药卫生—病原生物学]