干扰基本同源物增强子基因的表达对黑色素瘤细胞增殖、迁移和细胞周期的影响  

Effects of Interfering with the Expression of Enhancer of Rudimentary Homolog Gene on Proliferation,Migration and Cell Cycle of Melanoma Cells

作　　者：肖东兴 熊钰祯 贺新强[1] 赵冲 严朝晓 王传家[1] 赵明权[1] XIAO Dong-xing;XIONG Yu-zhen;HE Xin-qiang;ZHAO Chong;YAN Chao-xiao;WANG Chuan-jia;ZHAO Ming-quan(Department of Plastic Surgery,Shenzhen Longgang Central Hospital,Shenzhen 518116,China)

机构地区：[1]深圳市龙岗中心医院整形外科,广东深圳518116

出　　处：《南昌大学学报（医学版）》2023年第1期14-20,72,共8页Journal of Nanchang University：Medical Sciences

基　　金：广东省医学科研基金立项项目(A2020466)。

摘　　要：目的 探讨基本同源物增强子(enhancer of rudimentary homolog,ERH)基因敲除对恶性黑色素瘤(malignant melanoma,MM)细胞增殖、迁移和细胞周期的影响及机制。方法 采用免疫组织化学法检测ERH在MM组织和正常皮肤组织中的表达水平,并分析ERH的表达与MM患者临床病理参数的关系。sh-ERH转染MM细胞系,分为Lvsh-NC组和Lvsh-ERH组;CCK-8实验检测各组细胞的增殖能力;流式细胞仪检测各组细胞的周期比率和凋亡水平;Transwell实验检测各组细胞的迁移能力;Western blotting检测抑制ERH对MM细胞系中p21、CDK2、Cyclin E、Cleaved-Caspase-3、Bax和Bcl-2蛋白表达的影响。结果 与正常皮肤组织相比,ERH在MM组织中的表达上调(P<0.001),ERH表达与肿瘤分期和淋巴转移风险相关(P<0.05)。Lvsh-ERH转染MM细胞后,细胞的增殖和迁移能力均显著降低(P<0.001);与Lvsh-NC组相比,Lvsh-ERH组细胞凋亡率显著增加,且细胞周期阻滞在G0/G1期(P<0.01);转染Lvsh-ERH可显著增加MM细胞p21、Cleaved-Caspase-3和Bax蛋白表达,并降低CDK2、Cyclin E和Bcl-2表达(P<0.01)。结论 ERH在MM组织和细胞中均表达上调,抑制MM细胞中ERH的表达可能会降低其增殖、迁移和细胞周期转化能力,抑制MM的恶性进展。靶向ERH可能是治疗MM的潜在分子靶点。Objective To investigate the effects and mechanisms of enhancer of rudimentary homolog(ERH)knockdown on proliferation,migration and cell cycle of malignant melanoma(MM)cells.Methods The expression levels of ERH in MM tissues and normal skin tissues were detected by immunohistochemistry,and the relationship between ERH expression and clinicopathological parameters was analyzed in MM patients.MM cells transfected with sh-ERH were divided into Lvsh-NC group and Lvsh-ERH group.Cell proliferation was detected by CCK-8 assay.Cell cycle and apoptosis were determined by flow cytometry.The migration ability of cells was measured by Transwell assay.The effects of ERH inhibition on expression of p21,CDK2,cyclin E,cleaved caspase-3,Bax and Bcl-2 were detected by Western blotting.Results Compared with normal skin tissues,ERH expression was up-regulated in MM tissues(P<0.001).The expression of ERH was significantly associated with the tumor stage and the risk of lymphatic metastasis(P<0.05).The proliferation and migration abilities of cells were reduced after Lvsh-ERH transfection(P<0.001).Compared with the Lvsh-NC group,the apoptosis rate increased in the Lvsh-ERH group,and the cell cycle was blocked in the G0/G1 phase(P<0.01).Transfection with Lvsh-ERH increased p21,cleaved caspase-3 and Bax expression,and decreased CDK2,cyclin E and Bcl-2 expression in MM cells(P<0.01).Conclusion ERH expression is up-regulated in MM tissues and cells.The inhibition of ERH expression may reduce the proliferation,migration and cell cycle transition ability in MM cells and suppress the malignant progression of MM.Therefore,ERH may be a potential molecular target for the treatment of MM.

关 键 词：基本同源物增强子 恶性黑色素瘤 增殖 迁移 细胞周期 

分 类 号：R-33[医药卫生] R34