LINC01518对IL-17诱导非小细胞肺癌细胞增殖的影响  

Effects of LINC01518 on the proliferation of non-small cell lung cancer cells stimulated with IL-17

作　　者：阮玉婷 李雅 葛文 吴宁霞 应帅 王伟民 李玉[1] 张婧[1] 邱文[1] 王迎伟[1] 赵晨卉[2] RUAN Yuting;LI Ya;GE Wen;WU Ningxia;YING Shuai;WANG Weimin;LI Yu;ZHANG Jing;QIU Wen;WANG Yingwei;ZHAO Chenhui(Department of Immunology,Nanjing Medical University,Nanjing 211166;Department of Oncology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学免疫学系,江苏南京211166 [2]南京医科大学第一附属医院肿瘤科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2023年第3期304-310,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81902878,81971468)。

摘　　要：目的:探讨长链非编码RNA01518(long intergenic non-protein coding RNA 01518,LINC01518)基因过表达或沉默对白介素(interleukin,IL)-17诱导非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞增殖的影响。方法:Western blot检测NSCLC细胞系(PC9、H1299和H1975)IL-17受体A(IL-17 receptor A,IL-17RA)的表达后,用IL-17刺激H1299和PC9细胞不同时间,利用CCK-8实验测定细胞的增殖水平。LncRNA芯片筛查IL-17刺激H1299和PC9细胞3 h后LncRNA上调的结果,从中选取某些上调的LncRNA进行RT-PCR和Real-time PCR验证。此外,将构建的pcDNA3.1/LINC01518或shLINC01518质粒转染H1299细胞,用CCK-8、EdU和克隆形成实验检查细胞的增殖情况。结果:3种NSCLC细胞系均有IL-17RA的表达。实验发现,IL-17刺激H1299和PC9细胞后能提高其增殖水平,同时受刺激的细胞内LINC01518的上调最为显著,且过表达LINC01518可提高H1299细胞的增殖水平,而沉默LINC01518基因则能抑制IL-17诱导的细胞增殖。结论:LINC01518能促进IL-17诱导的H1299细胞增殖。Objective:This study aims to explore the effect of overexpressing or silencing long intergenic non-protein coding RNA 01518(LINC01518)on the cell proliferation induced by IL-17 in non-small cell lung cancer(NSCLC).Methods:The IL-17receptor A(IL-17RA)expression in NSCLC cell lines(PC9,H1299 and H1975)was first examined using Western blot.Then H1299and PC9 cells were exposed to IL-17 for different time,and the cell proliferation was detected by CCK-8 assay.Based on the results of up-regulated lncRNAs detected by lncRNA microarray in IL-17-stimulated H1299 and PC9 cells,several lncRNAs were selected and verified by RT-PCR and Real-time PCR.Additionally,the constructed plasmids of pcDNA3.1/LINC01518 or shLINC01518 were transfected into H1299 cells,and H1299 cell proliferation were examined by CCK-8,EdU and colony formation assays.Results:IL-17RA could express in 3 kinds of NSCLC cell lines.The cell proliferation and LINC01518 were obviously up-regulated in H1299and PC9 cells treated by IL-17.Besides,LINC01518 overexpression could markedly promote the cell proliferation,while LINC01518gene knockdown followed by IL-17 treatment did not enhance cell proliferation.Conclusion:LINC01518 can promote H1299 cell proliferation induced by IL-17 stimulation.

关 键 词：NSCLC IL-17 LINC01518 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]