超高效液相色谱-串联质谱法测定果蔬中4种乙撑二硫代氨基甲酸酯农药残留  

Determination of four ethylenebis dithiocarbamates in vegetables and fruits by UPLC-MS/MS

作　　者：杨昊 公丕学 王骏 王德利 薛霞 于文江 YANG Hao;GONG Pixue;WANG Jun;WANG Deli;XUE Xia;YU Wenjiang(Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Shandong Institute for Food and Drug Control,Shandong Ji’nan 250101,China)

机构地区：[1]山东省食品药品检验研究院,山东省食品药品安全检测工程技术研究中心,山东济南250101

出　　处：《中国食品卫生杂志》2023年第1期79-85,共7页Chinese Journal of Food Hygiene

摘　　要：目的建立超高效液相色谱-串联质谱法(UPLC-MS/MS)测定蔬菜、水果中4种乙撑二硫代氨基甲酸酯类农药残留量的方法。方法试样采用L-半胱氨酸盐酸盐和碱性EDTA-Na2溶液进行提取,碘甲烷衍生,QuEChERS原理净化浓缩,UPLC-MS/MS对乙撑二硫代氨基甲酸酯类农药衍生物进行测定,外标法定量。结果水果的检出限为5μg/kg,蔬菜的检出限为10μg/kg,相关系数大于0.998,添加回收率的范围为82.3%~115.1%,相对标准偏差为2.3%~6.9%。结论本文所建立的方法能够满足多种水果和蔬菜中乙撑二硫代氨基甲酸酯类农药快速准确检测。Objective A method of UPLC-MS/MS for the determination of four ethylenebis dithiocarbamates in vegetables and fruits was established.Methods Samples were extracted with lcysteine hydrochloride and alkaline EDTANa2 solutions,derived by methyliodide,prurified by QuEChERS method,and determined by UPLC-MS/MS.Results The limit of detection was 5μg/kg for fruits and 10μg/kg for vegetables.A good linear relationship was obtained between the peak area and concentration with the correlation coefficient over 0.998.The recoveries were from 82.3%to 115.1%,and the relative standard deviations were 2.3%-6.9%.Conclusion Ethylenebis dithiocarbamates in many kinds of vegetables and fruits could be successfully determined by the proposed method.

关 键 词：乙撑二硫代氨基甲酸酯类农药 残留 蔬菜、水果 UPLC-MS/MS QUECHERS 

分 类 号：R155[医药卫生—营养与食品卫生学]