固氮菌Azotobacter salinestris代谢物脂肪酸分析及其抗氧化活性研究  

Fatty Acid Analysis and Antioxidant Activity of Metabolites of Azotobacter salinestris

作　　者：付振艳[1] 排孜丽亚·帕尔哈提 宁慧霞[1] 牛立涛 热合巴提·努尔夏提 排合尔丁·穆太力甫 阿布力米提·伊力[1] FU Zhen-yan;Paziliya·Paerhati;NING Hui-xia(State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization/Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi,Xinjiang 830011;University of Chinese Academy of Sciences,Beijing 100039)

机构地区：[1]新疆特有药用资源利用省部共建国家重点实验室培育基地/中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [2]中国科学院大学,北京100039 [3]中国科学院新疆分院,新疆乌鲁木齐830011

出　　处：《安徽农业科学》2023年第5期172-175,共4页Journal of Anhui Agricultural Sciences

基　　金：中科院西部之光人才培养引进计划[E12SB11401(ARP)];中科院中亚药物研发中心科研项目(CAM202101)。

摘　　要：[目的]探究固氮菌Azotobacter salinestris(As101)代谢物脂肪酸组成及抗氧化活性。[方法]采用气质联用法(GC/MS)进行脂肪酸组成分析;根据2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基、1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟基自由基清除能力测定抗氧化活性。[结果]固氮菌As101代谢物脂溶性成分中棕榈酸甲酯(11.34%)、邻苯二甲酸二丁酯(7.41%)、顺9,10-甲基十九烷酸甲酯(18.71%)、硬脂酸甲酯(3.08%)、12-甲基十四烷酸甲酯(8.02%)为主要成分,其次为油酸甲酯(5.01%)、(Z)-十六烯酸甲酯(3.44%)、14-甲基十六烷酸甲酯(1.67%)等。其中,不饱和脂肪酸达43.78%;固氮菌As101代谢物脂肪酸对DPPH自由基、ABTS自由基及羟基自由基的清除能力分别为53.05%、27.83%、43.77%。[结论]As101代谢物脂肪酸种类丰富,具有良好的开发利用价值及广阔的应用前景。[Objective]The fatty acid composition and antioxidant activity of metabolites of Azotobacter salinestris(As101)were studied.[Method]The composition of fatty acids was analyzed by GC/MS;The antioxidant activity was determined according to the scavenging ability of 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt(ABTS),1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and hydroxyl radical.[Result]Palmitic acid(11.34%),phthalic acid(7.41%),cis-9,10-methyloctadecanoic acid(18.71%),stearic acid(3.08%),12 methyltetradecanoic acid(8.02%)were the main components of the fat soluble metabolites of Azotobacter As101,followed by oleic acid(5.01%),(z)-hexadecenic acid(1.44%),14 methylhexadecanoic acid(1.67%).Among them,unsaturated fatty acids reached 43.78%;the scavenging abilities of fatty acids of metabolites of nitrogen fixing bacteria AS101 to DPPH radical,ABTS radical and hydroxyl radical were 53.05%,27.83%and 43.77%respectively.[Conclusion]As101 metabolites are rich in fatty acids,which have good development and utilization value and broad application prospects.

关 键 词：固氮菌 代谢物 脂肪酸 GC/MS 抗氧化活性 

分 类 号：Q939.113[生物学—微生物学]