壳聚糖酶结构与功能的研究进展  

Advances in the structure and function of chitosanase

作　　者：谢杰 李玉斌 刘京伟 苟艳 王刚刚[1,2] XIE Jie;LI Yubin;LIU Jingwei;GOU Yan;WANG Ganggang(Key Laboratory of Environmental and Applied Microbiology of Chinese Academy of Sciences,Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;Key Laboratory of Environmental Microbiology of Sichuan Province,Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院成都生物研究所,中国科学院环境与应用微生物重点实验室,成都610041 [2]中国科学院成都生物研究所,环境微生物四川省重点实验室,成都610041 [3]中国科学院大学,北京100049

出　　处：《生物工程学报》2023年第3期912-929,共18页Chinese Journal of Biotechnology

基　　金：四川省科技厅计划项目(2022YFSY0028);中国科学院种子创新研究院项目;中国科学院环境与应用微生物重点实验室项目。

摘　　要：壳聚糖酶是一类对壳聚糖具有较高催化活性而几乎不水解几丁质的糖苷水解酶,其可将高分子量的壳聚糖转化为低分子量的功能性壳寡糖。近年来,对壳聚糖酶的相关研究取得了显著进展,因此,本文对其生化性质、晶体结构、催化机制和蛋白质工程改造进行总结和探讨,并对酶法制备壳寡糖纯品进行展望,这将加深研究者对壳聚糖酶作用机制的认识,推动壳聚糖酶的工业应用。Chitosanases represent a class of glycoside hydrolases with high catalytic activity on chitosan but nearly no activity on chitin. Chitosanases can convert high molecular weight chitosan into functional chitooligosaccharides with low molecular weight. In recent years,remarkable progress has been made in the research on chitosanases. This review summarizes and discusses its biochemical properties, crystal structures, catalytic mechanisms, and protein engineering, highlighting the preparation of pure chitooligosaccharides by enzymatic hydrolysis. This review may advance the understandings on the mechanism of chitosanases and promote its industrial applications.

关 键 词：壳聚糖酶 晶体结构 催化机制 理性设计 

分 类 号：Q55[生物学—生物化学]