原人参二醇型皂苷水解酶及制备人参皂苷Compound K的研究进展  

Protopanaxadiol-type ginsenoside hydrolases and their application in the preparation of ginsenoside Compound K:a review

作　　者：杨文华 顾秋亚 余晓斌[1,2] YANG Wenhua;GU Qiuya;YU Xiaobin(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214000,Jiangsu,China;School of Biotechnology,Jiangnan University,Wuxi 214000,Jiangsu,China)

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214000 [2]江南大学生物工程学院,江苏无锡214000

出　　处：《生物工程学报》2023年第3期978-992,共15页Chinese Journal of Biotechnology

基　　金：国家轻工技术与工程一流学科自主课题(LITE2018-11);江苏省研究生科研创新项目(SJCX19_0774)。

摘　　要：人参皂苷Compound K(CK)是一种具有抗癌抗炎等药理活性的化合物。目前在天然人参中暂未鉴定出,工业上主要通过原人参二醇型皂苷的去糖基化进行制备。相对于传统的物理、化学的去糖基化法,利用原人参二醇型皂苷水解酶制备CK具有特异性强、绿色环保和高效稳定的优点。本文根据水解酶作用的糖基连接碳原子的差异将原人参二醇型皂苷水解酶分成了3类,发现大多数能制备CK的水解酶为Ⅲ型原人参二醇型皂苷水解酶。此外,对水解酶在制备CK中的应用进行了总结评估,旨在为人参皂苷CK的大规模制备及其在食品和药品行业中的开发提供参考。Ginsenoside Compound K(CK)has anti-cancer and anti-inflammatory pharmacological activities.It has not been isolated from natural ginseng and is mainly prepared by deglycosylation of protopanaxadiol.Compared with the traditional physicochemical preparation methods,the preparation of CK by hydrolysis with protopanaxadiol-type(PPD-type)ginsenoside hydrolases has the advantages of high specificity,environmental-friendliness,high efficiency and high stability.In this review,the PPD-type ginsenoside hydrolases were classified into three categories based on the differences in the glycosyl-linked carbon atoms of the hydrolase action.It was found that most of the hydrolases that could prepare CK were PPD-type ginsenoside hydrolase typeⅢ.In addition,the applications of hydrolases in the preparation of CK were summarized and evaluated to facilitate large-scale preparation of CK and its development in the food and pharmaceutical industries.

关 键 词：原人参二醇型皂苷水解酶 酶催化 应用 制备 人参皂苷Compound K 

分 类 号：R284.3[医药卫生—中药学]