CDK4基因在肺癌中的表达及其意义  

The expression of CDK4 in lung cancer and its significance

作　　者：李保林 钟金琼 邹丽君[3] 胡恭华[3] 高艳芳[3] LI Bao-lin;ZHONG Jin-qiong;ZOU Li-jun;HU Gong-hua;GAO Yan-fang(School of Basic Medicine,Gannan Medical University;Department of Laboratory Medicine,The First Affiliated Hospital of Gannan Medical University;School of Public Health and Health Managemen,Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院基础医学院 [2]赣南医学院第一附属医院检验科 [3]赣南医学院公共卫生与健康管理学院,江西赣州341000

出　　处：《赣南医学院学报》2023年第1期1-4,9,共5页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(81903364);江西省教育厅科学技术研究项目(GJJ170862);赣南医学院校级重点项目和博士启动基金项目(QD201601、ZD201701)。

摘　　要：目的:研究CDK4基因在肺癌中的表达及其作用,为肺癌的治疗及预后提供依据。方法:⑴从TCGA数据库中获取肺癌数据,采用R软件对CDK4基因在肺癌中突变率进行统计学分析,并用卡方检验比较不同肺癌病理分型中CDK4基因突变率差异情况;⑵用配对t检验方法对癌组织跟癌旁组织CDK4基因差异表达进行比较;⑶对293例肺癌组织样本的CDK4 mRNA表达水平使用单因素Log-rank检验,分析CDK4 mRNA与肺癌无复发生存时间的关系,并用COX Regression model校正TNM分期因素后,再进一步对患者年龄进行相关性分析,对患者性别、T分期、N分期、M分期进行方差分析。结果:⑴肺鳞状细胞癌CDK4基因突变率为0.0014;肺腺癌CDK4基因突变率为0.04;小细胞肺癌CDK4基因突变率为0;Pan-Cancer CDK4基因突变率为0.04;采用卡方检验发现肺腺癌、肺鳞状细胞癌和小细胞肺癌中CDK4基因突变差异有统计学意义(P<0.01)。⑵配对t检验结果显示,与癌旁组织相比,CDK4基因在癌组织中显著上调(P<0.01)。⑶单因素Log-rank生存分析CDK4基因与预后关系表明,CDK4基因与肺癌预后有关(P<0.01)。⑷对CDK4基因与临床特征(TNM分期、性别)进行方差分析,结果显示,CDK4基因与患者性别、年龄、肿瘤远处转移无明显相关,CDK4基因与肿瘤淋巴结侵犯、肿瘤大小呈正相关(P均<0.01)。结论:不同病理分型肺癌中CDK4基因突变率不同,CDK4基因与肺癌发生、发展密切相关,可作为判断肺癌淋巴结转移、预后的一项新指标。Objective:To study the expression and function of CDK4 gene in lung cancer,and to provide basis for the treatment and prognosis of lung cancer.Methods:⑴Lung cancer data were obtained from the TCGA database,and R software was used to statistically analyze the mutation rate of CDK4 gene in lung cancer,and the chi-square test was used to compare the difference in the mutation rate of CDK4 gene in different pathological types of lung cancer;⑵With paired T test,the differential expression of CDK4 gene in cancer tissue and adjacent tissue was compared;⑶The CDK4 mRNA expression level of 293 lung cancer tissue samples was analyzed by single factor log-rank test to analyze CDK4.The relationship between mRNA and recurrence-free survival time of lung cancer was analyzed,and the COX Regression model was used to correct TNM staging factors,and then further correlation analysis was performed on patients’age,and variance analysis was performed on patient gender,T stage,N stage,and M stage.Results:⑴The mutation rate in lung squamous cell carcinoma was 0.0014;the mutation rate in lung adenocarcinoma was 0.04;the mutation rate in small cell lung cancer was zero;the pan-cancer mutation rate was 0.04;There was a statistically significant difference in CDK4gene mutation between squamous cell carcinoma and small cell lung cancer(P<0.01).⑵The results of paired T test showed that compared with adjacent tissues,CDK4 gene expression was significantly up-regulated in cancer tissues(P<0.01).⑶Univariate log-rank survival analysis showed that the relationship between CDK4 gene and prognosis showed that CDK4 gene was related to the prognosis of lung cancer(P<0.01).⑷Variance analysis was performed on CDK4gene and clinical characteristics(TNM stage,gender),and age correlation analysis was performed.The results showed that:CDK4 gene was positively correlated with tumor lymph node invasion and tumor size(P<0.01).Conclusion:The mutation rate of CDK4 gene in different pathological types of lung cancer is different.CDK4 gene is closel

关 键 词：肺癌 CDK4基因 预后分析 

分 类 号：R361.2[医药卫生—病理学]