A Meloidogyne incognita effector Minc03329 suppresses plant immunity and promotes parasitism  

作　　者：ZHOU Jing-jing ZHANG Xiao-ping LIU Rui LING Jian LI Yan YANG Yu-hong XIE Bing-yan ZHAO Jian-long MAO Zhen-chuan 

机构地区：[1]State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第3期799-811,共13页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(31672010 and 31871942);the Beijing Natural Science Foundation,China(6222054);the China Agriculture Research System(CARS-23);the Natural Science Foundation of Inner Mongolia Autonomous Region of China(2018MS03083)。

摘　　要：Meloidogyne incognita is a devastating plant-parasitic nematode.Effectors play important roles during the stages of nematodes infection and parasitism,but their molecular functions remain largely unknown.In this study,we characterized a new effector,Minc03329,which contains signal peptide for secretion and a C-type lectin domain.The yeast signal sequence trap experiments indicated that the signal peptide of Minc03329 is functional.In situ hybridization showed that Minc03329 was specifically expressed in the subventral esophageal gland.Real-time qPCR confirmed that the expression level of Minc03329 transcript was significantly increased in pre-parasitic and parasitic second-stage juveniles(pre-J2s and par-J2s).Tobacco rattle virus(TRV)-mediated gene silencing of Minc03329 in host plants largely reduced the pathogenicity of nematodes.On the contrary,ectopic expression of Minc03329 in Arabidopsis thaliana significantly increased plant susceptibility to nematodes.Transient expression of Minc03329 in Nicotiana benthamiana leaves suppressed the programmed cell death triggered by the pro-apoptotic protein BAX.Moreover,the transcriptome analysis of Minc03329-transgenic Arabidopsis and wild type revealed that many defense-related genes were significantly down-regulated.Interestingly,some different expressed genes were involved in the formation of nematode feeding sites.These results revealed that Minc03329 is an important effector for M.incognita,suppressing host defense response and promoting pathogenicity.

关 键 词：Meloidogyne incognita EFFECTOR C-type lectin PATHOGENICITY plant immunity 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]