基于HPLC检测方法的真菌毒素代谢规律研究  

Study on mycotoxin metabolism based on HPLC detection method

作　　者：周显青[1] 胡锋卫 曹宇飞[2] 张咚咚 赵金凤 赵锦琦 李梦瑶 ZHOU Xianqing;HU Fengwei;CAO Yufei;ZHANG Dongdong;ZHAO Jinfeng;ZHAO Jinqi;LI Mengyao(Engineering Research Center of Grain Storage and Security,Ministry of Education,Henan Provincial Engineering Technology Research Center on Grain Loss Reduction Post Harvest,School of Food and Strategic Reserves,Henan University of Technology,Zhengzhou 450001;Zhengzhou Depot Ltd Company of China Grain Reserves,Zhengzhou 450001)

机构地区：[1]粮食储藏与安全教育部工程研究中心,河南省粮食产后减损工程技术研究中心,河南工业大学粮食和物资储备学院,郑州450001 [2]中央储备粮郑州直属库有限公司,郑州450001

出　　处：《中国食品添加剂》2023年第3期1-8,共8页China Food Additives

基　　金：国家自然科学基金项目(32001745);中国科协第五届青年人才托举工程项目(2019QNRC001);河南工业大学高层次人才科研基金项目(2019BS011)。

摘　　要：玉米是我国主要的储备粮种之一,营养丰富且带菌量大,在收获、储藏等过程中,易发生霉变,产生大量真菌毒素,引发储粮安全问题。本文通过一次前处理实现对玉米样品中多种毒素的提取与检测,确定了AFTB_(1)、AFTB_(2)、AFTG_(1)、AFTG_(2)、ZEN、DON共6种玉米中常见真菌毒素的高效液相色谱检测方法。为了明确玉米储藏过程中真菌毒素代谢和温度的关系,我们将前期获得的两株产毒霉菌:黄曲霉和禾谷镰刀菌,接种于无菌玉米粉中,在22、28、38℃以及常温下储藏40 d后,监测其毒素含量变化。结果表明:温度对两种菌的真菌毒素代谢能力有较大的影响,较高的温度虽然会有利于霉菌的生长繁殖,但是其代谢产毒会受到抑制,较低的温度下,虽然霉菌的生长受到抑制,但也可能产生了大量的毒素。为更好的解析霉菌在玉米储藏过程中的产毒规律,确保储粮安全提供了数据支撑。Maize is one of the main grain reserve in our country since it is rich in nutrients. However,it is also easy to get a large amount of bacteria during the harvest or storage,such as mycotoxins which often causes the safety issue during the storage time. In this paper,the extraction and detection of various toxins in maize were carried out by one pretreatment. Six mycotoxins in maize of AFTB_(1),AFTB_(2),AFTG_(1),AFTG_(2),ZEN and DON were determined by HPLC. In order to study the effect of storage temperature on mycotoxins metabolism,two toxin-producing molds obtained in the previous stage,Aspergillus flavus and Fusarium graminearum were inoculated into sterile maize flour and stored at 22,28,38 ℃ and normal temperature respectively. After 40 days,changes in toxin content were tested.The results showed that the temperature had great influence on mycotoxin content. Higher temperature would benefit the growth of mold,but its toxin production would be inhibited. Lower temperature would inhibit the growth of mold,but large amounts of toxins would be produced. The study provides data to better analyze the mechanism of mold toxin production during maize storage and can help to ensure safety of stored maize.

关 键 词：高效液相色谱 储藏 温度 真菌毒素 储粮安全 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.3[轻工技术与工程—食品科学与工程]