大熊猫肠道乳酸菌分离鉴定及耐药表型和基因型分析  

Isolation of Intestinal Lactic Acid Bacteria from Giant Pandas and Analysis of Drug⁃Resistant Phenotypes and Genotypes

作　　者：王黎明 王宇翔 周杰珑 王洁 谢军金 张文平 WANG Liming;WANG Yuxiang;ZHOU Jielong;WANG Jie;XIE Junjin;ZHANG Wenping(Chengdu Research Base of Giant Panda,Chengdu 610081,China;Life Science College,Southwest Forestry University,Kunming 650224,China;Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife,Chengdu Research Base of Giant Panda,Chengdu 610081,China;Sichuan Academy of Giant Panda,Chengdu 610081,China)

机构地区：[1]成都大熊猫繁育研究基地,成都610081 [2]西南林业大学生命科学学院,昆明650224 [3]成都大熊猫繁育研究基地,四川省濒危野生动物保护生物学重点实验室,成都610081 [4]四川省大熊猫科学研究院,成都610081

出　　处：《四川动物》2023年第2期172-182,共11页Sichuan Journal of Zoology

基　　金：四川省科技计划项目应用基础研究项目(2019YJ0637);成都大熊猫繁育研究基金项目(CPF2017-11);成都大熊猫繁育研究基地项目(2020CPB-B13)。

摘　　要：通过培养法从健康大熊猫Aliuropoda melanoleuca新鲜粪便中分离乳酸菌,然后采用药敏纸片法(K-B法)和PCR对其进行耐药分析。共分离到79株乳酸菌,其对卡那霉素(99%)、磺胺异恶唑(99%)、多黏菌素B(90%)、阿米卡星(87%)、氨曲南(84%)、诺氟沙星(84%)、万古霉素(84%)、甲氧苄啶(81%)和环丙沙星(65%)的耐药率较高,对头孢类、青霉素类、四环素类、氯霉素类、大环内脂类、林克霉素类较敏感;其中15株检出耐药基因,1株携带2种耐药基因;共发现4种不同的耐药基因:四环素类tetM(检出率13.9%)和tetW(检出率3.8%)、β-内酰胺类bla TEM(检出率1.3%)、氯霉素类cat(检出率1.3%)。结果表明,大熊猫肠道乳酸菌对目前常用的抗生素敏感,可为大熊猫临床抗生素的合理用药提供科学依据,为大熊猫肠道疾病提供科学指导。Lactic acid bacteria were isolated from fresh feces of healthy giant pandas(Aliuropoda melanoleuca)by microbial culture techniques,and K-B method and PCR were used to analyze the drug resistance of these strains.The results showed that 79 strains of lactic acid bacteria were isolated and highly resistant to kanamycin(99%),sulfisoxazole(99%),polymyxin B(90%),amikacin(87%),aztreonam(84%),norfloxacin(84%),vancomycin(84%),trimethoprim(81%),and ciprofloxacin(65%),while sensitive to cephalosporins,penicillin,tetracycline,chloramphenicol,macrolides and lincomycinum.Four different drug resistance genes,including tetM(13.9%),tetW(3.8%),blaTEM(1.3%)and cat(1.3%)were detected in 15 strains,and 1 strain was found to harbor 2 drug-resistant genes.This study provides a reference for the rationality of clinical antibiotics and scientific guidance to improve the survival rate of giant pandas and intestinal diseases.

关 键 词：大熊猫 圈养 乳酸菌 耐药性 

分 类 号：Q959.8[生物学—动物学]