ML-9调节STIM1在下咽癌中的表达及作用机制  

ML-9 regulates the expression and mechanism of STIM1 in hypopharyngeal carcinoma

作　　者：孙源昊 李文龙 代兵 常江 于慧媛 常江 SUN Yuan-hao;LI Wen-long;DAI Bing(Sami Medical Center in Shenzhen,Shenzhen 518118,China)

机构地区：[1]深圳市萨米医疗中心,广东深圳518118

出　　处：《中国处方药》2023年第3期35-38,共4页Journal of China Prescription Drug

基　　金：深圳市萨米医疗中心院级科研基金(SSMC-2021-A15)。

摘　　要：目的分析ML-9调节STIM1在下咽癌中的作用机制。方法选取90只饲养裸鼠作为研究对象,应用随机法分为对照组及ML-9组,各45例。比较两组治疗前后细胞增殖、凋亡、侵袭、裸鼠体重、瘤体大小、细胞转移、蛋白表达量情况。结果治疗前,两组裸鼠各项指标比较,差异无统计学意义(P>0.05);治疗后,ML-9组裸鼠蛋白表达量、瘤体体积、瘤体重量情况均低于对照组,差异有统计学意义(P<0.05);ML-9组细胞增殖、凋亡、侵袭情况低于对照组,差异有统计学意义(P<0.05)。结论敲减下咽癌FaDu细胞中的STIM1基因后,下咽癌细胞的增殖、侵袭、转移能力也被显著抑制,并能够抑制下咽癌的细胞生物学行为,说明STIM1是调控下咽癌发生发展的关键因子。Objective To analyze the mechanism of ML-9 regulating STIM1 in hypopharyngeal carcinoma.Methods 90 nude mice were randomly divided into the control group and ML-9 group,45 cases in each group.The cell proliferation,apoptosis,invasion,body weight,tumor size,cell metastasis and protein expression of nude mice were compared between the two groups before and after treatment.Results Before treatment,there was no significant difference in all indexes between the two groups(P>0.05).After treatment,the protein expression,tumor volume and tumor weight of nude mice in ML-9 group were lower than those in the control group,the results were statistically significant(P<0.05).The cell proliferation,apoptosis and invasion in ML-9 group were lower than those in the control group,and the results were statistically significant(P<0.05).Conclusion After the knockdown of STIM1 gene in hypopharyngeal carcinoma FaDu cells,the proliferation,invasion and metastasis of hypopharyngeal cancer cells are also significantly inhibited,and the biological behavior of hypopharyngeal cancer cells can be inhibited,indicating that STIM1 is a key factor regulating the occurrence and development of hypopharyngeal cancer.

关 键 词：ML-9 STIM1 下咽癌 细胞增殖 蛋白表达量 生物学行为 

分 类 号：R739.63[医药卫生—肿瘤]