半滑舌鳎配合饵料发酵条件的优化及应用  

Optimization and application of conditions for fermented dietary of Cynoglossus semilaevis

作　　者：李璐 孙永智 陈玮 王丹 王世信 黄炳山 李培玉 王际英 LI Lu;SUN Yong-zhi;CHEN Wei;WANG Dan;WANG Shi-xin;HUANG Bing-shan;LI Pei-yu;WANG Ji-ying

机构地区：[1]山东省海洋资源与环境研究院,山东省海洋生态修复重点实验室,山东烟台264006

出　　处：《饲料研究》2023年第3期47-51,共5页Feed Research

基　　金：烟台市科技计划项目(项目编号:2021XDHZ056、2020YT06000121、2021XDHZ055)。

摘　　要：试验旨在研究3种益生菌复合发酵半滑舌鳎配合饵料的最适比例及时间。试验以半滑舌鳎配合饵料为底物,菌种总接种量为3%,35℃下进行酿酒酵母菌、枯草芽孢杆菌和植物乳杆菌的发酵处理,对照组未接种任何菌种。选取健康、平均体重(80.27±0.19) g的半滑舌鳎300尾,随机分为2组,每组3个重复,每重复50尾鱼。对照组半滑舌鳎饲喂配合饵料,试验组在配合饵料中添加10%发酵饵料(按照优化试验所得最适条件发酵饵料)。试验期56 d。结果显示,发酵后各组发酵饵料的活菌数均提高十万倍左右,3种菌种混合发酵效果显著优于对照组(P<0.05)。酿酒酵母菌∶枯草芽孢杆菌∶植物乳杆菌以1∶3∶2的比例发酵第5 d时发酵效果最优。与对照组相比,试验组半滑舌鳎体增重与特定生长率均显著提高(P<0.05)。研究表明,半滑舌鳎全价饵料在35℃下,以酿酒酵母菌∶枯草芽孢杆菌∶植物乳杆菌=1∶3∶2比例发酵5 d的效果较好,且发酵饵料可促进半滑舌鳎生长,提高饵料利用率。The experiment was to study the optimum proportion and time of compound feed for three kinds of probiotics.In the experiment,the feed of Cynoglossus semilaevis was used as substrate,the total inoculum amount was 3%,and the fermentation treatment of Saccharomyces cerevisiae,Bacillus subtilis and Lactobacillus plantarum was carried out at 35 ℃,while the control group was not inoculated with any inoculum.A total of 300 healthy Cynoglossus semilaevis with an average body weight of(80.27±0.19) g were randomly divided into two groups,with three replicates in each group and 50 Cynoglossus semilaevis in each replicate.The control group was fed with complex diet,and the experimental group was fed with complex diet supplemented with 10% fermented feed(fermented feed under the optimal conditions obtained in the optimization experiment).The experiment lasted for 56 d.The results showed that after fermentation,the number of live bacteria in each group of fermented food increased by about 100 000 times.The mixed fermentation effect of three strains was significantly better than that of control group(P<0.05).The fermentation effect was the best when the ratio of Saccharomyces cerevisiae∶ Bacillus subtilis∶ Lactobacillus plantarum was 1∶ 3∶ 2 for 5 d.Compared with control group,the body weight gain and specific growth rate of Cynoglossus semilaevis in test group were significantly higher(P<0.05).The experiment indicates that the fermentation effect of Saccharomyces cerevisiae∶ Bacillus subtilis∶Lactobacillus plantarum at a ratio of 1∶3∶2 for 5 d is better at 35 ℃,and the fermentation feed can promote the growth and improve the feed utilization rate of Cynoglossus semilaevis.

关 键 词：半滑舌鳎 配合饵料 生物发酵 酸溶蛋白 

分 类 号：S963.5[农业科学—水产养殖]