2019—2020山东小麦区试品系55个基因的 等位基因分布  

Allele Distribution of 55 Genes in Wheat Lines Participating in 2019-2020 Shandong Trial

作　　者：李玮[1] 张敏 宋国琦[1] 李玉莲[1] 高洁[1] 程敦公[1] 李豪圣[1] 李根英[1] LI Wei;ZHANG Min;SONG Guoqi;LI Yulian;GAO Jie;CHENG Dungong;LI Haosheng;LI Genying(Crop Research Institute,Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley,Ministry of Agriculture and Rural Afairs/National Engineering Laboratory for Wheat and Maize,Jinan,Shandong 250100;College of Agronony,Qingdao Agricultural University,Qingdao,Shandong 266109)

机构地区：[1]山东省农业科学院作物研究所/农业农村部黄淮北部小麦生物学与遗传育种重点实验室/小麦玉米国家工程实验室,山东济南250100 [2]青岛农业大学农学院,山东青岛266109

出　　处：《核农学报》2023年第1期17-26,共10页Journal of Nuclear Agricultural Sciences

基　　金：山东省农业良种工程(2019LZGC001);山东省重大科技创新工程(2020CXGC010805)。

摘　　要：为分析2019—2020年山东省小麦区试参试品系携带优异等位基因情况,利用产量、品质、抗性、开花期等55个基因的64个竞争性等位基因特异性聚合酶链式反应(KASP)标记对126份参试品系进行分子标记检测。结果表明,90%以上KASP标记具有较好的分型结果,可高效地进行基因型鉴定。20个基因的优异等位基因频率高于80%,包括Vrn-A1、Vrn-B1、Vrn-D1、Ppd-A1、Ppd-B1、Ppd-D1、Psy-D1、GluA3g、Rht-D1、Pinb-D1、TaCwi-A1-1、TaGW2-6B、TaSus1-7B、TaGASR7-A1、1-feh-w3、TaDreb-B1、PRRA1、PRR-B1、TaFT3-B1和TaMOT1-D1;26个基因的优异等位基因频率低于30%,包括Vp1-B1、Ppo-D1、TaPds-B1、Zds-A1、TEF-7A、Lr46、Glu-B3g、TaGS5-A1、TaCwi-4A、1B/1R、Rht-B1、Lr68、TaELF3-B1、Pina-D1、Sbwm1、TaPHS1、Pm21、COMT-3B、TaCKX-D1、TaSdr-B1、Pch1、Lr34、Yr15、Fhb1、TaMoc-7A和TaGS-D1,其中TaMoc-7A、Lr34、Fhb1、Pch1、Yr15和TaSdr-B1未检测到优异等位基因;9个基因的优异等位基因频率介于30%~80%,包括Pinb2-V、TaPod-A1、Lox-B1、Glu-A1、Glu-D1、TaGS2-B1、TaGW2-6A、TaSus1-7A和Lr14a。55个基因的优异等位基因型占比呈两极分化趋势。所有参试品系中仅济农CH03、CG086和济农CH01分别携带TaCKX-D1、Pm21和COMT-3B基因的优异等位基因,可作为基因供体用于粒重、抗白粉病和茎秆木质素含量改良和品种选育。本研究明确了126份参试品系的基因型信息,基本摸清了山东参试小麦品系在重要农艺性状基因上的等位基因分布特征,为分子标记辅助选择奠定了基础。To analyze gene distribution in wheat lines involved in 2019--2020 Shandong regional trial,one hundred and twenty-six wheat lines were tested with 64 Kompetitive Allele Specific polymerase chain reaction(KASP)markers which were specific to 55 genetic loci.The markers are related to yield,quality,resistance,flower time and etc.Over 90%KASP markers give acceptable genotyping,which provides an efficient technology for gene identification.Favorable allele frequencies of 20 genes are above 80%,including Vrn-A1,Vrn-B1,Vrn-D1,Ppd-A1,Ppd-B1,Ppd-D1,Psy-D1,Glu-A3g,Rht-D1,Pinb-D1,TaCwi-A1-1,TaGW2-6B,TaSus1-7B,TaGASR7-AI,1-feh-w3,TaDreb-B1,PRR-A1,PRR-B1,TaFT3-B1 and TaMOT1-D1.Favorable allele frequencies of 26 genes are below 30%,including Vp1-B1,Ppo-D1,TaPds-B1,Zds-A1,TEF-7A,Lr46,Glu-B3g,TaGS5-A1,TaCwi-4A,1B/1R,Rht-B1,Lr68,TaELF3-B1,Pina-D1,Sbwml,TaPHS1,Pm21,COMT-3B,TaCKX-D1,TaSdr-B1,Pch1,Lr34,Yr15,Fhb1,TaMoc-7A and TaGS-D1,notably,favorable alleles of TaMoc-7A,Lr34,Fhb1,Pch1,Yr15,and TaSdr-B1 are not detected.Favorable allele frequencies of 9 genes are between 30%and 80%,including Pinb2-V,TaPod-A1,Lox-B1,Glu-A1,Glu-D1,TaGS2-Bl,TaGW2-6A,TaSusl-7A,and Lr14a.Favorable allele frequencies of 55 genes show polarization trend.Among all tested lines,JinongCH03,CG086,and JinongCHO1 are the only one possessing favorable alleles of TaCKX-DI,Pm21,and COMT-3B,which could be used as gene donors for kernel weight,powdery mildew resistance,and stem lignin content improvement and breeding.In this study,genotypes of 55 genes in 126 wheat lines are clarified,allelic distribution of important agronomic genes in wheat lines attending the Shandong regional trial was basically clarified,which will serve as the foundation for marker assistant selection breeding.

关 键 词：小麦品系 分子标记 等位基因 区域试验 

分 类 号：S512.1[农业科学—作物学]