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作 者:郭锦堂 薄少波 张根林[1] GUO Jintang;BO Shaobo;ZHANG Genlin(School of Chemistry and Chemical Engineering,Shihezi University,Shihezi,Xinjiang 832003,China)
机构地区:[1]石河子大学化学化工学院,新疆石河子832003
出 处:《石河子大学学报(自然科学版)》2023年第1期80-86,共7页Journal of Shihezi University(Natural Science)
基 金:国家自然科学基金项目(22178226);兵团中青年科技创新领军人才计划项目(2017CB007)。
摘 要:为了探究硫酸盐对粘红酵母X-20(Rhodotorula glutinis X-20)富硒的影响,在恒定浓度亚硒酸钠和梯度浓度硫酸钠条件下培养R.glutinis X-20,研究硫酸盐对R.glutinis X-20生物量、总硒及硒纳米颗粒的影响,并使用RT-qPCR初步探索了硫酸盐调节R.glutinis X-20富硒的分子机制。研究发现,硫酸钠的添加使菌株的生物量提高了48.5%,总硒及硒纳米颗粒含量分别降低了36.7%和48.8%。RT-qPCR分析显示硫酸盐会抑制硒代谢关键基因CTH、metE、metB的表达,造成硫酸盐存在条件下总硒及硒纳米颗粒含量下降。硫酸盐的添加提高了R.glutinis X-20在亚硒酸钠环境中的生物量,这对富硒酵母产品的大规模生产具有重要意义。同时,RT-qPCR的结果也为R.glutinis X-20在分子生物学上的优化指明了方向。To investigate the effect of sulfate on the selenium enrichment of Rhodotorula glutinis X-20,the yeast was cultured under constant sodium selenite concentration and gradient sodium sulfate concentration to investigate the effect of sulfate on the biomass of the yeast,total selenium and selenium nanoparticles,and RT-qPCR was used to explore the preliminary mechanism of sulfate regulation of yeast selenium enrichment using RT-qPCR to explore the molecular mechanism of sulfate regulation.The addition of sodium sulfate was found to increase the biomass of the yeast strain by 48.5% and decrease the total content of selenium and selenium nanoparticle by 36.7% and 48.8%,respectively.RT-qPCR analysis revealed that sulfate suppressed the expression of CTH,metE,and metB,key genes for selenium metabolism,resulting in a decrease in the total content of selenium and selenium nanoparticles in the presence of sulfate.The addition of sulfate improved the biomass of R.glutinis X-20 in the sodium selenite environment,which is important for the large-scale production of selenium-enriched yeast products.Meanwhile,the results of RT-qPCR also indicated the direction for the optimization of R.glutinis X-20 in molecular biology.
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