Coordinated transcriptional and post-transcriptional epigenetic regulation during skeletal muscle development and growth in pigs  

作　　者：Du Zhang Shumei Wu Xinxin Zhang Shuqiang Ren Zhonglin Tang Fei Gao 

机构地区：[1]Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518000,China [2]College of Animal Sciences,South China Agricultural University,Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding,National Engineering Research Center for Breeding Swine Industry,Guangzhou 510642,China [3]Kunpeng Institute of Modern Agriculture at Foshan,Chinese Academy of Agricultural Sciences,Foshan 528226,China [4]GuangXi Engineering Centre for Resource Development of Bama Xiang Pig,Bama 547500,China [5]Comparative Pediatrics and Nutrition,Department of Veterinary and Animal Sciences,Faculty of Health and Medical Sciences,University of Copenhagen,Copenhagen,Denmark.

出　　处：《Journal of Animal Science and Biotechnology》2023年第2期564-577,共14页畜牧与生物技术杂志（英文版）

基　　金：supported by the Agricultural Science and Technology Innovation Program and The Elite Young Scientists Program of CAAS.ZT was supported by the National Natural Science Foundation of China(31830090);the Basic and Applied Basic Research Foundation of Guangdong province(2019B1515120059);the Shenzhen Dapeng New District Special Fund for Industry Development(KY20180114);the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202006).

摘　　要：Background:N6-methyladenosine(m^(6)A)and DNA 5-methylcytosine(5mC)methylation plays crucial roles in diverse biological processes,including skeletal muscle development and growth.Recent studies unveiled a potential link between these two systems,implicating the potential mechanism of coordinated transcriptional and post-transcrip-tional regulation in porcine prenatal myogenesis and postnatal skeletal muscle growth.Methods:Immunofluorescence and co-IP assays were carried out between the 5mC writers and m^(6)A writers to investigate the molecular basis underneath.Large-scale in-house transcriptomic data were compiled for applying weighted correlation network analysis(WGCNA)to identify the co-expression patterns of m^(6)A and 5mC regulators and their potential role in pig myogenesis.Whole-genome bisulfite sequencing(WGBS)and methylated RNA immu-noprecipitation sequencing(MeRIP-seq)were performed on the skeletal muscle samples from Landrace pigs at four postnatal growth stages(days 30,60,120 and 180).Results:Significantly correlated expression between 5mC writers and m^(6)A writers and co-occurrence of 5mC and m^(6)A modification were revealed from public datasets of C2C12 myoblasts.The protein-protein interactions between the DNA methylase and the m^(6)A methylase were observed in mouse myoblast cells.Further,by analyzing tran-scriptome data comprising 81 pig skeletal muscle samples across 27 developmental stages,we identified a 5mC/m^(6)A epigenetic module eigengene and decoded its potential functions in pre-or post-transcriptional regulation in postnatal skeletal muscle development and growth of pigs.Following integrative multi-omics analyses on the WGBS methylome data and MeRIP-seq data for both m^(6)A and gene expression profiles revealed a genome/transcriptome-wide correlated dynamics and co-occurrence of 5mC and m^(6)A modifications as a consequence of 5mC/m^(6)A crosstalk in the postnatal myogenesis progress of pigs.Last,we identified a group of myogenesis-related genes collaboratively regulated by both 5mC a

关 键 词：DNA methylation Epigenetic modification Epigenomic analysis 5mC regulators m^(6)A methylation m^(6)A regulators MYOGENESIS 

分 类 号：S813.1[农业科学—畜牧学]