三种罗汉松根际土壤微生物群落功能多样性  

Functional Diversity of Rhizosphere Soil Microbial Communities of Three Species of Pinus Koraiensis

作　　者：霍灿灿 朱栗琼[1] 招礼军[1] 甘远东 黄相玲 HUO Cancan;ZHU Liqiong;ZHAO Lijun;GAN Yuandong;HUANG Xiangling(Forestry College,Guangxi University/Guangxi Key Laboratory of Forest Ecology and Conservation,Nanning,Guangxi 530004,China)

机构地区：[1]广西大学林学院/广西森林生态与保育重点实验室,广西南宁530004

出　　处：《热带农业科学》2023年第2期1-7,共7页Chinese Journal of Tropical Agriculture

基　　金：国家自然科学基金(No.31560061)。

摘　　要：利用Biolog微平板技术,研究台湾大叶罗汉松、金钻罗汉松和罗汉松的不同土层深度根际土壤微生物碳代谢及微生物群落功能多样性的差异,分析罗汉松复杂的共生体与土壤环境的相关性,为罗汉松的栽培管理提供依据。结果表明,3种罗汉松根际土壤微生物对碳源的利用在平均颜色变化率(AWCD)指标中0~10 cm>10~20 cm>20~30 cm,其中罗汉松10~20 cm土层AWCD值最大,金钻罗汉松在20~30 cm土层中AWCD值最低;主成分分析表明,同种不同土层深度下,土壤微生物对碳源利用模式差异不显著,但不同种罗汉松在10~20 cm土层下微生物对碳源的利用模式差异显著,主要是糖类、羧酸类、氨基酸类三大碳源。在同种不同土层深度下Shannon多样性指数10~20 cm明显高于0~10 cm、20~30 cm土层,而Simpson优势度指数在各土层深度下差异不明显。说明10~20 cm土层是罗汉松根际土壤微生物的主要分布层,是微生物发挥根际效应的主要场所。Using Biolog microplate technology,the differences in soil microbial carbon metabolism and microbial community functional diversity in the rhizosphere at different soil depths of Podocarpus nakaii,P.annamiensis,and Podocarpus macrophyllus were studied,and the correlation between complex symbionts of Podocarpus and soil environment was analyzed,to provide the basis for the cultivation and management of Podocarpus.The results showed that the utilization of carbon source by three rhizosphere soil microorganisms of Podocarpus was 0-10 cm>10-20 cm>20-30 cm in the awcd index,among which the AWCD value of 10-20 cm soil layer of P.macrophyllus was the largest,and that of 20-30 cm soil layer of P.annamiensis was the lowest;principal component analysis showed that there was no significant difference in the use mode of carbon source by soil microorganisms under the same soil depth,but there was a significant difference in the use mode of carbon source by microorganisms under the 10-20 cm soil layer of different species of Luohansong,mainly including sugar,carboxylic acid,and amino acid.The Shannon diversity index of 10~20 cm is significantly higher than that of 0-10 cm and 20-30 cm soil layers at the same and different soil depths,while Simpson’s dominance index has no significant difference at each soil depth.It shows that the 10-20 cm soil layer is the prime distribution layer of soil microorganisms in the rhizosphere of Pinus grosvenorii,and it is the main place for microorganisms to exert the rhizosphere effect.

关 键 词：BIOLOG 罗汉松 主成份分析 碳源 土壤微生物 

分 类 号：S791.46[农业科学—林木遗传育种]