假小链双歧杆菌W112对免疫检查点阻断疗法的促进作用研究  

Promoting effect of Bifidobacterium pseudocatenulatum W112 on immune checkpoint blockade therapy

作　　者：吕梦欣 吴江涛 赵婧琦 贾龙刚 王艳萍[1] 耿伟涛 LYU Mengxin;WU Jiangtao;ZHAO Jingqi;JIA Longgang;WANG Yanping;GENG Weitao(College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457

出　　处：《食品与发酵工业》2023年第6期10-17,共8页Food and Fermentation Industries

基　　金：国家自然科学基金(31801514)。

摘　　要：该研究分析了一株假小链双歧杆菌W112对PD-1抗体免疫治疗的促进作用。首先通过在小鼠背部皮下注射B16-F10黑色素瘤细胞建立黑色素瘤小鼠模型,随后对肿瘤小鼠进行PD-1抗体治疗、灌胃假小链双歧杆菌W112及两者联合处理。结果表明,灌胃假小链双歧杆菌W112提高了PD-1抗体对肿瘤的抑制率,增加了小鼠的存活率,增加了肿瘤组织的CD8+T细胞浸润,并增加了肿瘤组织白细胞介素-12(interleukin-12,IL-12)和γ-干扰素(interferon-γ,IFN-γ)的富集。收集小鼠粪便并进行宏基因组测序分析发现,灌胃假小链双歧杆菌W112提高了小鼠肠道菌群的多样性,促进了有益菌的富集,降低了有害菌的富集。该研究表明,假小链双歧杆菌W112在促进免疫检查点疗法中具有一定的潜力。In this study,we analyzed the promoting effect of Bifidobacterium pseudocatenulatum W112 on PD-1 antibody immunotherapy.The melanoma mouse model was established by subcutaneous injection of B16-F10 melanoma cells into the back of mice.Then the tumor bearing mice were treated with PD-1 antibody,intragastric administration of B.pseudocatenulatum W112 and their combination.Results showed that intragastric administration of B.pseudocatenulatum W112 increased the tumor inhibition rate in PD-1 antibody treatment,including the increasing of survival rate of mice,infiltration of CD8+T cells in tumor tissues,and the enrichment of interleukin-12(IL-12)and interferon-γ(IFN-γ)in tumor tissues.The metagenome sequencing analysis of the microbiota in mice feces showed that intragastric administration of B.pseudocatenulatum W112 increased the diversity of mouse intestinal flora,promoted the enrichment of beneficial bacteria and reduced the enrichment of harmful bacteria.This study shows that B.pseudocatenulatum W112 has potential application in promoting immune checkpoint therapy.

关 键 词：假小链双歧杆菌W112 肠道菌群 黑色素瘤 免疫治疗 

分 类 号：R739.5[医药卫生—肿瘤]