FAM225A对卵巢癌细胞增殖和转移特性的影响  

Effect of FAM225A on proliferation and metastasis of cervical cancer cells

作　　者：孙桂霞[1] 张冬丽[1] 杨少琴[1] 曹芹雪[1] 田君[1] Sun Guixia;Zhang Dongli;Yang Shaoqin;Cao Qinxue;Tian Jun(Department of Gynecology,Huaihe Hospital of Henan University,Kaifeng 475000,China)

机构地区：[1]河南大学淮河医院妇瘤科,开封475000

出　　处：《中华实验外科杂志》2023年第1期127-130,共4页Chinese Journal of Experimental Surgery

基　　金：河南省医学科技攻关计划项目(LHGJ20210574)。

摘　　要：目的探讨非编码RNA FAM225A对卵巢癌Hela细胞增殖和转移特性的影响及其分子机制。方法选取2019年7月到2021年7月河南大学淮河医院收集的宫颈癌组织和癌旁组织作为研究对象,采用荧光定量聚合酶链反应(PCR)分析FAM225A表达水平。采用对照慢病毒和FAM225A短发卡RNA(shRNA)慢病毒感染人宫颈癌细胞系Hela,命名为对照组和FAM225A KD组,采用荧光定量PCR分析两组FAM225A表达水平;采用噻唑蓝(MTT)、克隆形成实验、流式细胞术、划痕实验、Transwell实验分析两组细胞的增殖、周期、迁移和侵袭能力。采用生物信息学和双荧光素酶报告基因分析FAM225A的靶基因及其下游调控基因。组间比较采用t检验。结果癌旁组织FAM225A表达水平(0.99±0.14)明显低于宫颈癌组织(2.05±0.23),差异有统计学意义(t=32.820,P<0.05)。对照组细胞吸光度值(2.02±0.14)明显高于FAM225A KD组(1.46±0.15),差异有统计学意义(t=6.591,P<0.05)。克隆形成实验结果显示,对照组细胞克隆形成率[(63.70±4.62)%]明显高于FAM225A KD组[(40.14±6.72)%],差异有统计学意义(t=7.077,P<0.05)。对照组细胞G0/G1期比例[(30.27±2.33)%]明显低于FAM225A KD组[(39.98±3.32)%],差异有统计学意义(t=7.077,P<0.05)。对照组细胞S期比例[(33.13±2.20)%]明显高于FAM225A KD组[(28.25±2.17)%],差异有统计学意义(t=3.866,P<0.05)。对照组细胞划痕愈合率[(81.17±5.71)%]明显高于FAM225A KD组[(63.17±6.40)%],差异有统计学意义(t=5.142,P<0.05)。对照组细胞侵袭数量[(105.17±14.82)个]明显高于FAM225A KD组[(70.18±10.32)个],差异有统计学意义(t=4.746,P<0.05)。miR-197-5p是FAM225A的海绵。癌旁组织miR-197-5p表达水平(1.07±0.15)明显高于宫颈癌组织(0.65±0.11),差异有统计学意义(t=19.350,P<0.05)。对照组细胞miR-197-5p表达水平(1.04±0.17)明显低于FAM225A KD组(1.81±0.31),差异有统计学意义(t=5.322,P<0.05)。结论FAM225A在宫颈癌组织中呈高表达,作为miR-197-Objective To investigate the effects of long non-coding RNA FAM225A on proliferation and metastasis of Hela cells and the molecular mechanism.Methods The cervical cancer tissues and adjacent tissues collected in our hospital from July 2019 to July 2021 were selected as the research objects and the expression level of FAM225A was analyzed by fluorescent quantitative polymerase chain reaction(PCR).Human cervical cancer cell line Hela was infected with control lentivirus and FAM225A short hairpin RNA(shRNA)lentivirus,named as control group and FAM225A KD group respectively.The expression level of FAM225A in the two groups was analyzed by fluorescence quantitative PCR.The proliferation,cycle,migration and invasion of cells in the two groups were analyzed by methyl thiazolyl tetrazolium(MTT),clone formation test,flow cytometry,scratch test and Transwell test.Bioinformatics and double luciferase reporter genes were used to analyze the target gene and its downstream regulatory gene of FAM225A.The measurement data between groups were compared by t test.Results The expression level of FAM225A in para-cancerous tissues(0.99±0.14)was significantly lower than that in cervical cancer tissues(2.05±0.23,t=32.820,P<0.05).The absorbance value of cells in the control group(2.02±0.14)was significantly higher than that in the FAM225A KD group(1.46±0.15,t=6.591,P<0.05).The rate of cell clone formation in the control group[(63.70±4.62)%]was significantly higher than that in the FAM225A KD group[(40.14±6.72)%,t=7.077,P<0.05].The proportion of cells in G0/G1 phase in the control group[(30.27±2.33)%]was significantly lower than that in the FAM225A KD group[(39.98±3.32)%,t=7.077,P<0.05].The proportion of cells in S phase in the control group[(33.13±2.20)%]was significantly higher than that in the FAM225A KD group[(28.25±2.17)%].The scratch healing rate of cells in the control group[(81.17±5.71)%]was significantly higher than that in the FAM225A KD group[(63.17±6.40)%,t=5.142,P<0.05].The number of invasive cells in the control

关 键 词：FAM225A 微小RNA 宫颈癌 增殖 迁移 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]