马银花中1个新的木脂素苷  

A new lignan glucoside from stems and branches of Rhododendron ovatum

作　　者：马敏 庾石山[1] MA Min;YU Shi-shan(State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China)

机构地区：[1]中国医学科学院,北京协和医学院药物研究所,天然产物活性物质与功能国家重点实验室,北京100050

出　　处：《中国中药杂志》2023年第2期415-420,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重点项目(21732008)。

摘　　要：采用硅胶、ODS、Sephadex LH-20凝胶、大孔树脂以及半制备高效液相色谱等技术,从马银花茎枝的95%乙醇提取物中分离得到10个木脂素类化合物,经UV、IR、HR-ESI-MS、ECD和NMR等多种波谱学方法鉴定其结构分别为(-)-4-表-南烛木树脂酚-9′-O-α-L-鼠李糖苷(1)、(+)-南烛木树脂酚-3α-O-α-L-鼠李糖苷(2)、(+)-5′-methoxyisolariciresinol-9′-O-α-L-鼠李糖苷(3)、(-)-南烛木树脂酚-3α-O-β-D-葡萄糖苷(4)、(+)-南烛木树脂酚-3α-O-β-D-葡萄糖苷(5)、(-)-4-表-南烛木树脂酚-9′-O-β-D-葡萄糖苷(6)、racemiside(7)、新刺五加酚(8)、(+)-丁香脂素(9)和homohesperitin(10)。其中,化合物1为1个新的芳基四氢萘型木脂素苷类化合物。以上化合物在体外的Fe^(2+)-半胱氨酸(Cys)诱导的肝微粒体脂质过氧化模型评价中,化合物8和9在1×10^(-5)mol·L^(-1)时,对Fe^(2+)-Cys诱导大鼠肝微粒体丙二醛(MDA)生成抑制效果显著,抑制率分别为75.20%、91.12%。Ten lignans were isolated from the ethanol extract of stems and branches of Rhododendron ovatum through column chromatography over silica gel,ODS,Sephadex LH-20,and MCI-gel resin and semi-preparative RP-HPLC.The structures of all compounds were elucidated by extensive spectroscopic data analysis(UV,IR,HR-ESI-MS,ECD and NMR)as(-)-4-epi-lyoniresinol-9′-O-α-L-rhamnopyranoside(1),(+)-lyoniresinol-3α-O-α-L-rhamnopyranoside(2),(+)-5′-methoxyisolariciresinol-9′-O-α-L-rhamnopyranoside(3),(-)-lyoniresinol-3α-O-β-D-glucopyranoside(4),(+)-lyoniresinol-3α-O-β-D-glucopyranoside(5),(-)-4-epi-lyoniresinol-9′-O-β-D-glucopyransoide(6),racemiside(7),neociwujiaphenol(8),(+)-syringaresinol(9),and homohesperitin(10).Among them,compound 1 was a new aryltetralin-type lignan.All the isolated lignans were tested for antioxidant activities in Fe^(2+)-cysteine induced rat liver microsomal lipid peroxidation in vitro,and compounds 8 and 9 showed antioxidant activities on the formation of malondiadehyde(MDA)in rat liver microsomes at 1×10^(-5)mol·L^(-1),with significant inhibitory rates of 75.20%and 91.12%,respectively.

关 键 词：马银花 木脂素类 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]