老年人晚发性抑郁症预后与外周血lncRNA表达水平和应对方式的关系  

Relationship between the prognosis of late-onset depression in the elderly and lncRNA expression levels in peripheral blood and coping styles

作　　者：孔令明 牛威 朱晓丽 陈升东 张理义 石海峰 Kong Lingming;Niu Wei;Zhu Xiaoli;Chen Shengdong;Zhang Liyi;Shi Haifeng(Mental Disease Prevention&Treatment Center,N0.904 Hospital of the Joint Logistic Support Force,Changzhou 213003,China;Department of Neurology,N0.904 Hospital of the Joint Logistic Support Force,Changzhou 213003,China;Department of Medical Imaging,the Second People's Hospital of Changzhou Affiliated to Nanjing Medical University,Changzhou 213003,China)

机构地区：[1]联勤保障部队第904医院心理中心,常州213003 [2]联勤保障部队第904医院神经内科,常州213003 [3]南京医科大学附属常州第二人民医院医学影像科,常州213003

出　　处：《中华老年医学杂志》2023年第3期267-271,共5页Chinese Journal of Geriatrics

基　　金：江苏省社会发展重点项目(BE2015615)。

摘　　要：目的 探讨老年人晚发性抑郁症(LOD)预后与lncRNA表达水平和应对方式的关系。方法 病例对照研究。实时荧光定量核酸扩增检测系统(qPCR)检测LOD 92例患者和80例对照组外周血差异表达lncRNA,并进行汉密顿抑郁量表(HAMD)、特质应对方式问卷(TCSQ)评估。结果 与对照组比较,老年人LOD TCONS_00019174(7.55比4.36)、ENST00000566208(6.48比3.26)、ENST00000517573(8.33比5.32)、NONHSAT142707(6.78比3.26)的表达水平更低(Z=5.09、5.87、4.35、6.44,均P<0.05或0.01);与低表达组比较,lncRNA高表达组的焦虑/躯体化[(3.83±1.40)比(6.39±2.35)]、日夜变化[(0.22±0.42)比(0.83±0.94)]、迟滞[(5.74±0.96)比(6.48±1.28)]、绝望感[(2.78±0.67)比(4.52±1.56)]、HAMD[(20.39±1.75)比(26.83±4.88)]分数更低(t=-4.50、-2.84、-2.22、-4.90、-5.96,均P<0.05)。TCONS_00019174的ΔCT值与焦虑/躯体化、日夜变化、迟滞、睡眠障碍、绝望感、HAMD减分率呈负相关(r=-0.40~-0.66,均P<0.05或0.01);ENST00000566208的ΔCT值与焦虑/躯体化、睡眠障碍、绝望感、HAMD减分率呈负相关(r=-0.47~-0.62,均P<0.01);ENST00000517573、NONHSAT034045、NONHSAT142707的ΔCT值与迟滞、睡眠障碍、绝望感、HAMD减分率呈负相关(r=-0.39~-0.76,均P<0.05);积极应对方式与HAMD总分及焦虑/躯体化、迟滞、睡眠障碍、绝望感因子减分率呈正相关(r=0.38~0.55,均P<0.05);消极应对方式与HAMD总分及焦虑/躯体化、睡眠障碍、绝望感因子减分率呈负相关(r=-0.39~-0.67,均P<0.05)。TCONS_00019174、ENST00000566208、NONHSAT034045、NONHSAT142707、积极应对和消极应对方式进入HAMD总减分率的回归方程,对HAMD总减分率有显著预测作用(t=-8.713、-3.584、-3.864、-2.257、5.675、-2.357,均P<0.05),可HAMD解释总减分率方差变异32.4%。结论 TCONS_00019174、ENST00000566208、NONHSAT034045、NONHSAT142707、积极应对方式、消极应对方式是老年人LOD预后的预测因子。Objective To investigate the correlation between the prognosis of late-onset depression(LOD)in the elderly and lncRNA expression levels and coping styles.Methods Differential expression of lncRNAs in peripheral blood of LOD 92 patients was detected by a real-time quantitative PCR(qPCR)detection system,and the Hamilton Depression Scale(HAMD)and the Trait Coping Style Questionnaire(TCSQ)were used for psychological assessment.Results Compared with the control group,the expression levels of TCONS_00019174(7.55 vs.4.36),ENST00000566208(6.48 vs.3.26),ENST00000517573(8.33 vs.5.32)and NONHSAT142707(6.78 vs.3.26)in elderly patients of the LOD group were significantly down-regulated(Z=5.09,5.87,4.35,6.44,P<0.05);Compared with the low-expression subgroup,scores of anxiety/somatization[(3.83±1.40)vs.(6.39±2.35)],diurnal variation[(0.22±0.42)vs.(0.83±0.94)],retardation[(5.74±0.96)vs.(6.48±1.28)],hopelessness[(2.78±0.67)vs.(4.52±1.56)]and HAMD[(20.39±1.75)vs.(26.83±4.88)]in the high-expression subgroup were significantly lower(t=-4.50,-2.84,-2.22,-4.90,-5.96,P<0.05).TheΔCT value of TCONS_00019174 was negatively correlated with the reduction rates of anxiety/somatization,diurnal variation,retardation,sleep disturbance,hopelessness and HAMD(r=-0.40-0.66,P<0.05).TheΔCT value of ENST00000566208 was negatively correlated with the reduction rates of anxiety/somatization,sleep disturbance,hopelessness and HAMD(r=-0.47-0.62,P<0.01).TheΔCT values of ENST00000517573,NONHSAT034045 and NONHSAT142707 were negatively correlated with the reduction rates of retardation,sleep disturbance,hopelessness and HAMD(r=-0.39-0.76,P<0.05).The positive coping style was positively correlated with the reduction rates of HAMD,anxiety/somatization,retardation,sleep disturbance and hopelessness(r=0.38-0.55),while the negative coping style was negatively correlated with the reduction rates of HAMD,anxiety/somatization,sleep disturbance and hopelessness(r=-0.39-0.67,P<0.05).When TCONS_00019174,ENST00000566208,NONHSAT034045,NONHSAT142707,positive c

关 键 词：抑郁 长链非编码RNA 应对方式 预后 

分 类 号：R749.4[医药卫生—神经病学与精神病学]