枸杞多糖对睡眠剥夺大鼠血液中T细胞亚群及相关炎性因子的影响  

Effects of Lycium barbarum polysaccharides on T cell subtypes and related inflammatory factors in blood of sleep deprived rats

作　　者：栾和芝[1] 单铁强[2] 姜柳[2] 韩长海 祁皎 吕晶 LUAN He-zhi;SHAN Tie-qiang;JIANG Liu;HAN Chang-hai;QI Jiao;LYU Jing(Laboratory Department,Port Hospital of Hebei Port Group Co.,Ltd,Qinhuangdao Hebei,066000,China;Department of Laboratory,Qinhuangdao Haigang Hospital,Qinhuangdao Hebei,066000,China;Medical College,Hebei Engineering University,Handan Hebei,056002,China)

机构地区：[1]河北港口集团有限公司港口医院检验科,河北秦皇岛066000 [2]秦皇岛市海港医院检验科,河北秦皇岛066000 [3]河北工程大学医学院,河北邯郸056002

出　　处：《职业与健康》2023年第3期317-321,共5页Occupation and Health

摘　　要：目的 分析枸杞多糖(Lycium barbarum polysaccharides,LBP)对睡眠剥夺大鼠血液中T细胞亚群型的数量、相关炎性因子以及大鼠行为能力的影响,探讨LBP对睡眠剥夺大鼠的免疫保护机制。方法 将SD大鼠通过“多平台水环境”技术成功制备剥夺睡眠大鼠模型,按照干预方法将其均等分成3组(模型组、LBP干预组及西药干预组),每组6只,同时把6只健康同种属大鼠作为对照组。在建模成功后4组鼠每天的干预用药不同,LBP干预组:灌服LBP(0.54 mg/kg),西药干预组:灌服舒乐安定(6 g/kg),模型组和对照组:灌服等容量三蒸水,持续用药4周。检测血液中T淋巴细胞亚群CD3+T细胞、CD4+T细胞、CD8+T细胞的比例以及CD4+T/CD8+T的比值;ELISA检测血液中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、γ-干扰素(interferon-γ,IFN-γ)、白介素-1β(interleukin-1β,IL-1β)、白介素-2(interleukin-2,IL-2)及白介素-6(interleukin-6,IL-6)的含量;检测各组大鼠的行为学指标以衡量其大脑学习记忆功能的变化。结果 和对照组相比,模型组大鼠血液中CD4+T细胞比例减少[(25.64±2.53)%],CD8+T细胞比例增多[(20.71±2.21)%],CD4+T/CD8+T比值(1.66±0.13)降低,血液中的炎性因子:TNF-α[(15.99±2.02)ng/mL]、IFN-γ[(3.22±0.45)ng/mL]、IL-1β[(22.70±2.87)pg/mL]、IL-2[(15.85±1.77)ng/mL]及IL-6[(64.65±6.23)pg/mL]的含量均增加,大鼠的行为能力[(42.36±4.60)s,(10.26±1.68)次]升高(均P<0.01);和模型组相比,LBP干预组大鼠血液中CD4+T细胞比例升高[(35.63±3.00)%]、CD8+T细胞比例降低[(14.10±1.94)%],CD4+T/CD8+T比值(2.38±0.21)增加,血液中的炎性因子:TNF-α[(10.25±1.72)ng/mL]、IFN-γ[(2.13±0.34)ng/mL]、IL-1β[(16.26±1.80)pg/mL]、IL-2[(11.90±1.50)ng/mL]及IL-6[(46.74±4.39)pg/mL]的含量均下降,大鼠的行为能力[(31.51±3.98)s,(16.90±2.72)次]降低(均P<0.05);LBP干预组与西药干预组相比,上述指标均没有明显差异(均P>0.05)。结论 LBP能够通过调节大鼠血液中TObjective To analyze the effects of Lycium barbarum polysaccharide (LBP) on the number of T cell subtypes,related inflammatory factors and behavioral ability of sleep deprived rats,explore the immune protective mechanism of LBP on sleep deprived rats.Methods SD rats were successfully prepared into sleep deprivation rat models by"multiplatform water environment"technology.According to the intervention methods,they were equally divided into three groups (model group,LBP intervention group and western medicine intervention group) with six rats in each group,at the same time,six healthy rats of the same genus were used as the control group.After successful modeling,the intervention drugs of the four groups were different every day.LBP intervention group was given LBP (0.54 mg/kg) orally,western medicine intervention group was given estazolam (6g/kg) orally,and model group and control group were given equal volume three distilled water orally,continuous medication for four weeks.The number of T lymphocyte subsets CD3+T cells,CD4+T cells,and the ratio of CD4+T/CD8+T in the blood were detected.The contents of tumor necrosis factor-α (TNF-α),γ-interferon (IFN-γ),interleukin-1β (IL-1β),interleukin-2 (IL-2) and interleukin-6(IL-6) in blood.The behavioral indicators of rats in four groups were tested to measure the changes of brain learning and memory function.Results Compared with the control group,the percentage of CD4+T cells in the blood of model group decreased[(25.64±2.53)%],the percentage of CD8+T cells increased[(20.71±2.21)%],the ratio of CD4+T/CD8+T decreased(1.66±0.13),the contents of the inflammatory factor in the blood increased,which included TNF-α[(15.99±2.02)ng/mL],IFN-γ[(3.22±0.45)ng/mL],IL-1β[(22.70±2.87)pg/mL],IL-2[(15.85±1.77)ng/mL]and IL-6[(64.65±6.23)pg/mL],and the behavioral ability of rats[(42.36±4.60)s,(10.26±1.68)times]increased(all P<0.01).Compared with the model group,the percentage of CD4+T cells in the blood of LBP intervention group increased[(35.63±3.00)%],the percentage o

关 键 词：枸杞多糖 睡眠剥夺 T细胞亚群 炎性因子 行为能力 

分 类 号：R-332[医药卫生]