分级醇沉竹荪多糖的结构及抗氧化活性比较  

Comparison of Structural and Antioxidant Activity of Dictyophora indusiata Polysaccharides Precipitated by Gradient Ethanol

作　　者：韩伟 卜原玲 姚泽远 王承潇[3] HAN Wei;BU Yuanling;YAO Zeyuan;WANG Chengxiao(Engineering Reseach Center of Pharmaceutical Process Chemistry,Ministry of Education,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;Faculty of Life Science and Technology,Kunming Unviersity of Science and Technology,Kunming 650000,China)

机构地区：[1]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海200237 [2]华东理工大学药学院上海市新药设计重点实验室,上海200237 [3]昆明理工大学生命科学与技术学院,云南昆明650000

出　　处：《徐州工程学院学报（自然科学版）》2023年第1期61-70,共10页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

基　　金：国家自然科学基金(82060645);上海市科技兴农重点攻关项目(沪农科创字(2018)第1-1号)。

摘　　要：通过缓冻协同微波法提取竹荪多糖,以20%~80%乙醇分级制备得到4种多糖组分,分别为PI-20、PI-40、PI-60和PI-80;采用UV、FI-IR、HPSEC-MALLS-RI、HPAEC对多糖结构进行分析.结果表明4种组分均含有少量蛋白质,但未检测到糖醛酸存在,PI-20和PI-40多糖含量均在50%以上;低浓度乙醇醇沉的多糖PI-20和PI-40的相对分子质量达到千万级,而高体积分数乙醇醇沉的多糖相对分子质量在万级水平;20%~60%醇沉多糖均由Gal、Glu和Man构成,PI-80仅由Glu和Man组成.4种醇沉多糖组分均具有一定的抗氧化能力,在3种抗氧化试验中,4种醇沉组分PI-80综合抗氧化能力最佳,清除ABTS自由基半抑制质量浓度为0.2484 mg/mL,PI-40综合抗氧化活性最弱,清除ABTS自由基半抑制质量浓度为2.9043 mg/mL.The polysaccharide of Dictyophora indusiata was extracted by slow freezing and microwave method.Four polysaccharide components,PI-20,PI-40,PI-60 and PI-80,were prepared respectively by fractionation with 20%~80%ethanol.UV,FI-IR,HPSEC-MALLS-RI,HPAEC were used to analyze the structure of polysaccharide.The results showed that the four components contained a small amount of protein,but no uronic acid was detected,and the content of PI-20 and PI-40 polysaccharides was more than 50%.The molecular weight of PI-20 and PI-40 precipitated by low concentration ethanol reached tens of millions,while the molecular weight of polysaccharide precipitated by high concentration ethanol reached ten thousand.20%~60%ethanol precipitation polysaccharide was composed of Gal,Glu and Man,and PI-80 was only composed of Glu and Man.All the four ethanol precipitated polysaccharides had certain antioxidant capacity.Among the three antioxidant tests,PI-80 had the best comprehensive antioxidant activity with the IC_(50) of 0.2484 mg/mL for scavenging ABTS free radicals,while PI-40 had the weakest antioxidant activity with the IC_(50) of 2.9043 mg/mL for scavenging ABTS free radicals.

关 键 词：竹荪多糖 分级醇沉 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]