山东省滨州市某社区来源产ESBLs大肠埃希菌及mcr-1阳性菌株耐药基因分析  

Analysis on drug resistance genes of ESBLs-producing Escherichia coli and mcr-1 positive strains from a community in Binzhou,Shandong

作　　者：韩佳运 王爽[2] 寇增强[2] 王安阁 马丽梅 刘璐 陈玉贞[2] 孙高翔 毕振旺 Han Jiayun;Wang Shuang;Kou Zengqiang;Wang Ange;Ma Limei;Liu Lu;Chen Yuzhen;Sun Gaoxiang;Bi Zhenwang(Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,Shandong,China;Shandong Center for Disease Control and Prevention,Jinan 250014,Shandong,China)

机构地区：[1]山东第一医科大学附属省立医院,山东济南250021 [2]山东省疾病预防控制中心,山东济南250014

出　　处：《疾病监测》2023年第1期64-69,共6页Disease Surveillance

基　　金：山东省重点研发计划(No.2017GSF218062);山东省医药卫生科技发展计划(No.2017WS447,No.202112071042);国家科技重大专项(No.2018ZX10713003-002)。

摘　　要：目的分析城市社区居民肠道来源产超广谱β-内酰胺酶(ESBL)大肠埃希菌和多黏菌素耐药基因mcr-1阳性菌株的分子流行病学特征。方法本研究对山东省滨州市某城市社区居民粪便样本中分离的16株产ESBL大肠埃希菌进行药物敏感性试验、全基因组测序(WGS)分析和单核苷酸多态性分析(SNPS)。对多黏菌素耐药的产ESBL大肠埃希菌进行S1-PFGE和Southern杂交确定耐药基因的位置,并用接合试验判断基因的可转移性。结果某城市社区居民肠道来源产ESBL大肠埃希菌的检出率为40.00%,多重耐药菌占75.00%。WGS分析显示,16株ESBL大肠埃希菌携带多种耐药基因、毒力基因。SNPS分析显示16株产ESBL大肠埃希菌聚集成4个簇。mcr-1阳性产ESBL大肠埃希菌携带的耐药基因mcr-1和blaCTX-M均位于质粒上,且均可随质粒发生水平转移。结论城市社区居民中可检出多黏菌素耐药ESBL大肠埃希菌,基因mcr-1存在水平传播的可能性。Objective To analyze the molecular epidemiological characteristics of extended-spectrumβ-lactamasesproducing Escherichia coli(ESBL-EC)and polymyxin resistance gene mcr-1 positive strains from the intestinal tract samples of urban community residents.Methods In this study,drug susceptibility test,whole genome sequencing(WGS)s and single nucleotide polymorphisms(SNPs)analysis were performed for 16 ESBL-EC strains isolated from the stool samples of residents in an urban community of Binzhou,Shandong province.S1-PFGE and Southern hybridization were performed for ESBL-EC which were resistant to polymyxin to identify the locus of the drug resistance gene.In addition,the conjugation experiment was conducted to determine the gene transferability.Results The detection rate of ESBL-EC from the intestinal tract samples of the community residents was 40.00%,and the multidrug-resistance rate was 75.00%.WGS showed that 16 strains of ESBL-EC carried multiple drug resistance genes and virulence genes.SNPs analysis showed that 16 strains of ESBLEC were divided into 4 clusters.The drug resistance genes,mcr-1 and blaCTX-M carried by mcr-1 positive ESBL-EC were all in the plasmids and could transfer horizontally with the plasmids.Conclusion Polymyxin resistant ESBL-EC and its resistance genes could be detected in urban community residents.Gene mcr-1 has the possibility of horizontal transmission.

关 键 词：大肠埃希菌 超广谱Β-内酰胺酶 全基因组测序 耐药基因 mcr-1 分子流行病学 

分 类 号：R211[医药卫生—中医学] R378