油莎豆种质资源苗期耐盐性鉴定与评价  

Identification and evaluation of salt tolerance of Tigernut germplasm resources at seedling stage

作　　者：于馥榕 李毅丹[2] 程云清[1] 杨向东[2] 任伟[2] YU Furong;LI Yidan;CHENG Yunqing;YANG Xiangdong;REN Wei(School of Life Science,Jilin Normal University,Siping,Jilin 136000,China;Agricultural Biotechnology Institute,Jilin Academy of Agricultural Sciences,Changchun,Jilin 130124,China)

机构地区：[1]吉林师范大学生命科学学院,吉林四平136000 [2]吉林省农业科学院,吉林长春130124

出　　处：《干旱地区农业研究》2023年第2期1-10,85,共11页Agricultural Research in the Arid Areas

基　　金：国家重点研发计划项目(2019YFC0507601,2019YFC0507602)。

摘　　要：选取国内外油莎豆(Cyperus esculeutus L.)种质资源39份作为试验材料,采用水培法,在1.2%NaCl胁迫条件下对其进行盐胁迫处理,测定17个幼苗生长相关指标,并利用主成分分析(PCA)、聚类分析、隶属函数等方法综合评价油莎豆种质资源耐盐性并筛选耐盐种质。结果表明:不同油莎豆种质资源各项指标的变异系数范围是0.128~0.455,其中,叶宽、枯黄率、根长、地上部鲜质量、地下部鲜质量等存在极显著差异(P<0.01);叶绿素和分蘖数存在显著差异(P<0.05)。通过主成分分析将17个指标转变成5个相互独立的综合指标,综合贡献率达到80.11%。干质量、叶绿素、茎粗、根冠比、分蘖数和组织含水量可作为主要耐盐评价指标。聚类分析将39份油莎豆种质划分为3个等级,依据CDC值及D值的分析结果,最终筛选出耐盐性强的油莎豆种质资源3份:JYD-35、JYD-40和JYD-3。The salt tolerance of thirty-nine germplasm resources of Tigernut(Cyperus esculeutus L.)from China and abroad were evaluated by measuring 17 physiological indicators using principal component analysis(PCA),cluster analysis,membership function and other methods under the normal and 1.2%NaCl treatment conditions.The results showed that the coefficient of variation of each indicator ranged from 0.128 to 0.455.All germplasms showed highly significant differences(P<0.01)in leaf width,yellowing rate,root length,fresh weight of aboveground and underground parts;and significant differences(P<0.05)in chlorophyll content and tiller number between the NaCl treatment and the normal conditions.According to the result of PCA,17 variables was transformed into 5 independent comprehensive indicators whose contribution rate reached 80.11%.Dry weight,chlorophyll content,stem diameter,root-shoot ratio,tiller number and tissue water content could be the main evaluation indicators for salt tolerance.All germplasms were clustered into 3 grades.According to the analysis results of CDC value and D value,three salt-tolerant germplasms of JYD-35,JYD-40 and JYD-3 were identified.

关 键 词：油莎豆 种质 幼苗期 耐盐性 筛选 

分 类 号：S565[农业科学—作物学] S32