Vitamin E modulates androgen receptor gene expression to attenuate ovarian dysfunctions in a rat model of dehydroepiandrosterone-induced polycystic ovary  

作　　者：Olugbemi T Olaniyan Ayobami Dare Charles O.Adetunji Gloria E.Okotie Joseph B.Dare Bosun M.Adigun Femi Adebayo 

机构地区：[1]Laboratory for Reproductive Biology and Developmental Programming,Department of Physiology,Edo University Uzairue,Edo State,Nigeria [2]Discipline of Physiology,School of Laboratory Medicine and Medical Sciences,College of Health Sciences,University of KwaZulu-Natal,Westville Campus,Durban,South Africa [3]Applied Microbiology,Biotechnology and Nanotechnology Laboratory,Department of Microbiology,Edo University Uzairue,Edo State,Nigeria [4]Department of Physiology,University of Ibadan,Ibadan,Nigeria [5]Department of Anatomy,Osun State University Oshogbo,Nigeria [6]Department of Physiology,Bingham University,Nasarawa State,Nigeria

出　　处：《Asian pacific Journal of Reproduction》2023年第2期81-89,共9页亚太生殖杂志（英文版）

摘　　要：Objective:To investigate the protective effect of vitamin E in dehydroepiandrosterone(DHEA)-induced polycystic ovary in rats.Methods:Premature female Wistar rats were randomly allocated into four groups,with 7 rats in each group.Group栺received corn oil(vehicle)and served as the control group;group栻received 0.2 mL of 0.06 mg/g DHEA in corn oil;group栿received 200 mg/kg vitamin E;group桇received DHEA plus vitamin E.All treatments lasted for 15 days,with DHEA administered subcutaneously,while vitamin E and corn oil were administered orally.After the experiment,serum samples and ovaries were harvested for biochemical,immunohistochemical,hormonal,and histological analysis.The ovarian mRNA expression of androgen receptor was analyzed by reverse transcriptase quantitative polymerase chain reaction(qPCR).Results:The antioxidant and metabolic enzyme activity significantly decreased in the DHEA-treated rats compared to the control rats(P<0.05).Administration of vitamin E to DHEAtreated rats significantly decreased cytokines and malondialdehyde compared to the DHEA-treated rats.The histological analysis showed reduced atretic and cystic ovaries,increased E-cadherin and Bcl-2 expression,and reduced expression of Bax in the DHEAtreated rats co-treated with vitamin E.The mRNA expression of androgen receptor was upregulated in the DHEA-treated rats compared to the control rats.Conclusions:Vitamin E ameliorates the hyperandrogenic effect of DHEA-induced polycystic ovaries via metabolic,antioxidant,and anti-apoptotic pathways.

关 键 词：DEHYDROEPIANDROSTERONE Metabolic markers Polycystic ovarian syndrome Vitamin E Wistar rats 

分 类 号：R711.75[医药卫生—妇产科学]