Genetic variation in GmCRP contributes to nodulation in soybean(Glycine max Merr.)  

作　　者：Jinhui Wang Chao Ma Shengnan Ma Haiyang Zheng Huilin Tian Xi Wang Yue Wang Hongwei Jiang Jiangxu Wang Zhanguo Zhang Chunyan Liu Mingliang Yang Qingshan Chen Dawei Xin 

机构地区：[1]Key Laboratory of Soybean Biology of Chinese Ministry of Education,Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China [2]Jilin Academy of Agricultural Sciences,Changchun 130033,Jilin,China [3]Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China

出　　处：《The Crop Journal》2023年第2期332-344,共13页作物学报（英文版）

基　　金：supported by the China Postdoctoral Science Foundation(2020M681072);the National Natural Science Foundation of China(32072014,U20A2027,and 31771882);‘Hundred-thousand and million project of Heilongjiang province for engineering and technology science’soybean breeding technology innovation and new cultivar breeding(2019ZX16B01).

摘　　要：Symbiosis between soybean and rhizobia contributes to soybean yield and quality. Although secreted rhizobial type Ⅲ effectors are known to regulate infection and promote nitrogen fixation, much about them remains unknown. Mutation of NopC, a type Ⅲ effector from Sinorhizobium fredii HH103, reduced nodule numbers and dry weights in 310 soybean accessions, and expression of NopC in soybean hairy roots promoted symbiosis. Based on observed differences in nodule traits between Suinong 14 and Zyd 00,006inoculated with HH103 and the NopC mutant, 11 QTL associated with rhizobia were identified in chromosome segment substitution lines(CSSLs) derived from Suinong 14 and Zyd 00006. Using chromosome fragment insertion, whole-genome sequencing of Suinong 14 and Zyd 00006, and qRT-PCR,Glyma.19G176300(GmCRP) was identified as a candidate gene associated with NopC, and GmCRP was found to be induced by NopC to positively regulate nodulation. SNPs located in the regulatory regions of GmCRP influenced its expression response to NopC, with SNPs contributing to nodulation having been selected during domestication. Our findings reveal the function of a soybean gene encoding a rhizobial type Ⅲ effector that contributes to symbiosis, and will facilitate the practical application of symbiotic nitrogen fixation in molecular breeding.

关 键 词：SOYBEAN SYMBIOSIS EFFECTOR NopC Natural varieties 

分 类 号：S565.1[农业科学—作物学]