Lin28B和C-myc蛋白在喉鳞癌中的表达及其与临床病理特征和预后的相关性  

Correlation between expression of Lin28B and C-myc in patients with laryngeal squamous cell carcinoma and clinicopathological features and prognosis

作　　者：廖非非 曹雨鑫 黄美响 初令[1] 王天生[2] LIAO Feifei;CAO Yuxin;HUANG Meixiang;CHU Ling;WANG Tiansheng(Department of Pathology,Third Xiangya Hospital,Central South University,Changsha 410013;Department of Otolaryngology Head and Neck Surgery,Third Xiangya Hospital,Central South University,Changsha 410013,China)

机构地区：[1]中南大学湘雅三医院病理科,长沙410013 [2]中南大学湘雅三医院耳鼻喉头颈外科,长沙410013

出　　处：《中南大学学报（医学版）》2023年第2期165-171,共7页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(82270003);长沙市自然科学基金(kq2202429)。

摘　　要：目的:喉鳞癌是头颈部常见恶性肿瘤。将原癌基因与抑癌基因作为研究的突破口从而筛选恶性肿瘤治疗的靶基因是如今肿瘤研究焦点之一。寻找喉鳞癌治疗及预后相关的靶基因分子至关重要。本研究旨在检测Lin28B和C-myc蛋白在喉鳞癌及其对应癌旁组织中的表达,初步探讨其与喉鳞癌临床病理特征及预后的关系。方法:采用免疫组织化学法检测102例喉鳞癌患者的癌组织及其对应的90例癌旁组织中Lin28B与C-myc蛋白的表达情况,分析Lin28B与C-myc蛋白在喉鳞癌中表达的相关性及其表达水平与喉鳞癌临床病理特征之间的关系。采用Kaplan-Meier法分析喉鳞癌患者术后生存率与Lin28B和C-myc蛋白表达水平之间的关系。结果:与癌旁组织相比,Lin28B、Cmyc蛋白的表达水平在喉鳞癌组织中均显著升高(均P<0.05)。且二者在喉鳞癌中表达存在正相关关系(r=0.476,P<0.05)。Lin28B蛋白的表达与喉鳞癌患者年龄、淋巴结转移情况、临床分期、肿瘤大小和病理分化程度密切相关(均P<0.05)。C-myc蛋白的表达与喉鳞癌患者淋巴结转移情况、临床分期、肿瘤大小和病理分化程度密切相关(均P<0.05)。生存分析显示高表达Lin28B(P=0.001)或C-myc蛋白(P<0.001)的患者术后生存率较低,差异有统计学意义。结论:Lin28B及C-myc蛋白在喉鳞癌组织中高表达且呈正相关,两者均与患者的淋巴结转移情况、临床分期、肿瘤大小、病理分化程度及预后相关,提示Lin28B和C-myc可能都参与喉鳞癌的发生、发展。Objective: Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor ofhead and neck. Screening of target genes for malignant tumor therapy is one of the focusesof cancer research, with proto-oncogene and tumor suppressor gene as the breakthrough. Ithas become an urgent need to find the target gene related to the treatment and prognosis ofLSCC.This study aims to explore the role of Lin28B and C-myc in LSCC by detecting theexpressions of these two proteins and analyze the correlation between the expression ofLin28B and C-myc and clinicopathological features and prognosis of LSCC.Methods: We detected the expression of Lin28B and C-myc proteins in 102 specimens ofLSCC and 90 specimens of adjacent tissues by immunochemistry, and analyzed thecorrelation between Lin28B and C-myc protein expressions in LSCC as well as thecorrelation between the expressions of the two proteins and the clinicopathological featuresof LSCC. At the same time, the Kaplan-Meier method was used to analyze the relationbetween Lin28B and C-myc protein levels with the postoperative survival rate of LSCCpatients.Results: The protein levels of Lin28B and C-myc in the LSCC tissnes were significantlyhigher than those in the adjacent tissues (both P<0.05),and there was a positive correlationbetween the expression of Lin28B and C-myc in LSCC (r=0.476, P<0.05). The expressionof Lin28B protein was closely related to age, lymph node metastasis, clinical stage, tumorsize, and pathological differentiation of LSCC patients (all P<0.05). while the expression ofC-myc protein was closely related to lymph node metastasis, clinical stage, tumor size, andpathological differentiation of LSCC patients (all P<0.05). A relevant survival analysisshowed that in patients with higher level of Lin28B (P=0.001) or C-myc protein (P<0.001), the postoperative survival rate was relatively low.Conclusion: Lin28B and C-myc proteins are highly expressed in LSCC with a positivecorrelation. Furthermore, they are closely related to lymph node metastasis, clinical stage,tumo

关 键 词：Lin28B C-MYC 喉鳞癌 临床病理特征 预后 

分 类 号：R739.65[医药卫生—肿瘤]