Bacillus and microalgae biofertilizers improved quality and biomass of Salvia miltiorrhiza by altering microbial communities  

作　　者：Xuemin Wei Xuanjiao Bai Pei Cao Gang Wang Jianping Han Zheng Zhang 

机构地区：[1]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China

出　　处：《Chinese Herbal Medicines》2023年第1期45-56,共12页中草药（英文版）

基　　金：supported by grants from the CAMS Innovation Fund for Medical Sciences (CIFMS, No. 2021-I2M-1-071)。

摘　　要：Objective: Biofertilizers are reliable alternatives to chemical fertilizers due to various advantages. However, the effect of biofertilizers on Salvia miltiorrhiza yield and quality and the possible mechanisms remain little known. Here, an experiment was conducted in S. miltiorrhiza field treated with two kinds of biofertilizers including Bacillus and microalgae.Methods: A field experiment was conducted on S. miltiorrhiza of one year old. The biofertilizers were applied at six treatments:(i) control check, CK;(ii) microalgae, VZ;(iii) Bacillus, TTB;(iv)microalgae + Bacillus(1:1), VTA;(v) microalgae + Bacillus(0.5:1), VTB;(vi) microalgae+Bacillus(1:0.5),VTC. Here, high-throughput sequencing, ICP-MS and UPLC were employed to systematically characterize changes of microbial diversity and structure composition, heavy metals content and bioactive compounds, respectively.Results: Compared to CK, root biomass increased by 29.31%-60.39%(P < 0.001). Meanwhile, bioactive compounds were higher than CK after the application of the biofertilizers, peculiarly in TTB and VTB.However, the content of Pb contents in roots significantly reduced by 46.03% and 37.58% respectively in VTC and TTB(P<0.05). VTA application notably increased the available nitrogen content by 53.03%(P<0.05), indicating the improvement of soil fertility. Significantly, bacterial and fungal Chao I diversity indices showed an increasing trend with biofertilizer application(P < 0.05), and biofertilizer amendment enriched the rhizosphere soil with beneficial microorganisms that have abilities on promoting plant growth(Achromobacter and Penicillium), adsorbing heavy metal(Achromobacter and Beauveria), controlling plant pathogen(Plectosphaerella, Lechevalieria, Sorangium, Phlebiopsis and Beauveria) and promoting the accumulation of metabolites(Beauveria and Phoma).Conclusion: Bacillus and microalgae biofertilizers improved the quality and biomass of S. miltiorrhiza by altering microbial communities in soil.

关 键 词：BIOFERTILIZER plant growth QUALITY rhizosphere microorganisms Salvia miltiorrhiza(Bge.) 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]