基于生物信息学分析核糖体蛋白S7与乳腺癌预后和免疫浸润的关系  

Bioinformatics-based analysis on the relationship between RPS7 and breast cancer pprognosis and immune infiltration

作　　者：方倩倩 沈国双[1] 解琪琪 赵福星 任登峰 刘震[1] 王淼舟[1] 赵久达[1] Fang Qianqian;Shen Guoshaung;Xie Qiqi;Zhao Fuxing;Ren Dengfeng;Liu Zhen;Wang Miaozhou;Zhao Jiuda(Affiliated Hospital of Qinghai University&Affiliated Cancer Hospital of Qinghai University,Xining 810001,China)

机构地区：[1]青海大学附属医院,青海大学附属肿瘤医院,西宁810001

出　　处：《中国高原医学与生物学杂志》2023年第2期132-144,共13页Journal of Chinese High Altitude Medicine & Biology

基　　金：青海省中央财政地方科技发展引进资金项目。

摘　　要：目的基于生物信息学分析核糖体蛋白S7(RPS7)与乳腺癌预后和免疫浸润的关系。方法从TCGA数据库获取乳腺癌表达谱数据及相关临床数据,然后使用TCGA、UALCAN、HPA、STRING、TIMER数据库和Kaplan-Meier法分析RPS7与乳腺癌预后和免疫浸润的关系。结果RPS7在乳腺癌中低表达,并与年龄和雌激素受体(Estrogen Receptor,ER)状态、孕激素受体(Progesterone Receptor,PR)状态及PAM50显著相关。RPS7高表达的患者与预后不良显著相关。通过功能富集分析表明,RPS7的生物学功能主要富集在角质化作用方面,分子功能主要富集于肽链内切酶抑制剂活性方面。经KEGG分析发现,RPS7可能影响神经组织的交互作用和蛋白质的消化吸收途径。此外,RPS7表达与B细胞、树突状细胞、调节性T细胞、CD8+T细胞和T细胞的免疫浸润水平呈显著正相关。结论RPS7可能为一种新的与乳腺癌预后和免疫浸润相关的生物标志物。Objective Bioinformatics-based analysis of ribosomal protein S7(RPS7)in relation to breast cancer prognosis and immune infiltration.Methods Breast cancer expression profile data and related clinical profile data were obtained from the TCGA database,and then the expression and role of RPS7 in breast cancer were analyzed using TCGA,UALCAN,HPA,STRING,TIMER databases and Kaplan-Meier.Results RPS7 was lowly expressed in breast cancer and significantly correlated with age,Estrogen Receptor(ER)status,Progesterone Receptor(PR)status and PAM50.Patients with high RPS7 expression were significantly associated with poor prognosis.Functional enrichment analysis showed that the biological functions of RPS7 were mainly enriched in keratinization and the molecular functions were mainly enriched in peptide chain endonuclease inhibitor activity.Analysis by KEGG revealed that RPS7 may affect neural tissue interactions and protein digestion and absorption pathways.In addition,RPS7 expression was significantly and positively correlated with the level of immune infiltration of B cells,dendritic cells,regulatory T cells,CD8+T cells and T cells.Conclusions RPS7 may be a new biomarker associated with breast cancer prognosis and immune infiltration.

关 键 词：RPS7 乳腺癌 生物标记物 预后 免疫浸润 生物信息学 

分 类 号：R735.2[医药卫生—肿瘤]