‘京红久’忍冬叶绿体基因组微卫星序列特征分析  

Characteristic Analysis of Microsatellite Sequences in Lonicera×heckrottii'Gold Flame'Based on Chloroplast Genome

作　　者：张加强 史小华 刘慧春 许雯婷 周江华 朱开元 Zhang Jiaqiang;Shi Xiaohua;Liu Huichun;Xu Wenting;Zhou Jianghua;Zhu Kaiyuan(Zhejiang Institute of Landscape Plants and Flowers,Hangzhou,311251)

机构地区：[1]浙江省园林植物与花卉研究所,杭州311251

出　　处：《分子植物育种》2023年第6期1955-1966,共12页Molecular Plant Breeding

基　　金：浙江省农科院新成果示范推广项目(tg2021009);浙江省农科院重大课题主持类项目(2020R25R08E03);萧山区重大科技计划项目(2020219)共同资助。

摘　　要：‘京红久’忍冬(Lonicera×heckrottii’Gold Flame’)是一种极具观赏价值的优良藤本,被誉为“最帅的攀援植物”。本研究利用前期测定的‘京红久’忍冬叶绿体基因组序列结果,应用生物信息学软件对其微卫星的分布特征进行了全面分析。结果发现:‘京红久’忍冬叶绿体基因组共有168个微卫星位点,相对丰度和相对密度分别为1 080.82 loci/Mb和10 280.69 loci/Mb,主要分布于大单拷贝区(large single copy regions, LSC)。在微卫星的碱基组成中,以单碱基微卫星为主(占总数的63.10%),其次是三碱基微卫星(占总数的29.17%),二碱基微卫星的数量最少(占总数的2.98%),未检测到五碱基重复序列和六碱基重复序列。同时,以A/T重复为主,占单碱基微卫星的61.90%。在微卫星重复次数中,单碱基微卫星位点序列的重复次数集中在8~17之间,三碱基微卫星的重复次数分布在4~5之间。相关性分析结果表明,微卫星重复序列的数量与长度、相对丰度和相对密度呈极显著正相关。不同物种的叶绿体基因组微卫星重复类型相差较大。本研究结果为‘京红久’忍冬的物种鉴定、系统分类、微卫星标记的开发以及引物筛选提供了理论依据。Lonicera×heckrottii'Gold Flame'is an excellent vine with great ornamental value and is known as"The most handsome of the climbing honeysuckles".In this study,the chloroplast genome sequence of Lonicera×heckrottii'Gold Flame'was determined in the previous study,and the distribution characteristics of chloroplast microsatellites were comprehensively analyzed by bioinformatics software.The results showed that there were 168 microsatellite loci on the chloroplast genome of Lonicera×heckrottii'Gold Flame',with relative abundance and relative density of 1080.82 loci/Mb and 10280.69 loci/Mb,respectively,mainly distributed in large single copy regions(LSC).In the base composition,mononucleotide repeat microsatellite were the most(accounting for 63.10%of the total),followed by trinucleotide repeat microsatellite(accounting for 29.17%of the total),and the number of dinucleotide repeat microsatellite was the least(accounting for 2.98%of the total),pentanucleotide and hexanucleotide repeat were not detected.At the same time,A/T repeats dominated,accounting for 61.9%of mononucleotide repeat microsatellite.Among the repeat times of microsatellites,the repeat times of mononucleotide microsatellite site sequences was concentrated between 8~17, and the repeat times of trinucleotide repeat microsatellite were between4~5. The results of correlation analysis showed that the number of microsatellite repeats was very significantlypositively correlated with length, relative abundance and relative density. Repeat types of microsatellites inchloroplast genomes of different species are different. The results of this study provide a theoretical basis for speciesidentification, systematic taxonomy, extensive development of microsatellite markers and primer screening forLonicera×heckrottii 'Gold Flame'.

关 键 词：‘京红久’忍冬 叶绿体基因组 微卫星 分布特征 

分 类 号：S685.99[农业科学—观赏园艺]