三链染色质结构R-loop的研究进展:从检测方法到生物学功能  

Recent progress on R-loop biology:from detection methods to biological functions

作　　者：周劲聪 王文杰 孙前文[1,2] ZHOU JinCong;WANG WenJie;SUN QianWen(Center for Plant Biology,School of Life Sciences,Tsinghua University,Beijing 100084,China;Tsinghua-Peking Center for Life Sciences,Beijing 100084,China)

机构地区：[1]清华大学生命科学学院植物生物学中心,北京100084 [2]清华大学-北京大学生命科学联合中心,北京100084

出　　处：《中国科学：生命科学》2023年第3期289-303,共15页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:32170321,31822028,91940306)资助。

摘　　要：R-loop是由一条单链DNA和另一条RNA∶DNA杂合链组成的三链染色质结构,在众多生物体中发挥着重要的生物学功能.生物体内有众多调控因子通过调节R-loop的稳态来影响各水平的基因组调控事件,如转录、复制、DNA损伤及修复等.近年来,随着R-loop的检测技术日益成熟,越来越多的R-loop生物学新功能正在被发现.本文归纳比较了不同的R-loop检测方法,并对植物R-loop生物学功能研究进展进行了重点阐述.R-loops are three-stranded chromatin structures composed of a single-stranded DNA and an RNA:DNA hybrid with important biological functions in numerous organisms.Numbers of R-loop regulators have been found to affect genomic events,such as transcription,replication,DNA damage,and repair,through modulating R-loop homeostasis.Over the last few years,with the development of R-loop detection methods,an increasing number of R-loop biological functions are being discovered.In this review,we provide an overview of R-loop detection methods and focus on the research progress of R-loop functions in plants.

关 键 词：RNA:DNA杂合链 R-LOOPS 基因组调控 

分 类 号：Q343.2[生物学—遗传学]