体外组装的病毒样颗粒在疫苗和药物递送中的应用  

Application of Virus Like Particles Assembled in Vitro in Vaccine and Drug Delivery

作　　者：李岩异 吕娜 贾思凝 张永红 张红霞 张卫婷 陈金利 LI Yanyi;LYU Na;JIA Sining;ZHANG Yonghong;ZHANG Hongxia;ZHANG Weiting;CHEN Jinli(North China Pharmaceutical Jintan Biotechnology Co.,Ltd.,Shijiazhuang 050035,China)

机构地区：[1]华北制药金坦生物技术股份有限公司,石家庄050035

出　　处：《生物技术进展》2023年第2期201-209,共9页Current Biotechnology

基　　金：河北省重点研发项目(21372403D)。

摘　　要：病毒样颗粒(virus like particles,VLPs)近年来被广泛应用于疫苗和治疗性药物递送系统领域。与体内组装VLPs相比,体外组装VLPs具有组装寡聚体成分明确、反应条件可控、避免宿主成分的引入以及能实现药物活性成分的包裹和递送功能等诸多优势。概述了体外VLPs的生产方法、影响体外VLPs形成的因素、VLPs的特征分析方法,综述了体外VLPs在疫苗和治疗性药物递送方面的应用进展,期望对体外VLPs组装技术的发展提供参考思路,促进体外VLPs组装技术在疾病预防和治疗应用方面发挥更大的作用。Virus like particles(VLPs)have been widely used in the field of vaccines and therapeutic drug delivery systems in recent years.Compared with VLPs assembled in vivo,VLPs assembled in vitro have many advantages,such as clear composition of assembled oligomers,controllable reaction conditions,avoiding the introduction of host components,and realizing the encapsulation and delivery of drug active components.This review mainly described the production methods of extracellular VLPs,the influencing factors of VLPs formation,and the characterization methods of VLPs assembled in vitro.The application of VLPs assembled in vitro in vaccine and therapeutic drug delivery was also reviewed.It is expected to provide reference ideas for the development and progress of VLPs assembled in vitro assembly technology,and promote VLPs assembled in vitro assembly technology to play a greater role in disease prevention and treatment.

关 键 词：衣壳蛋白 体外组装VLPs 病毒样颗粒 药物递送 

分 类 号：Q815[生物学—生物工程] R914.2[医药卫生—药物化学]