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作 者:奚天益 杨国荣[1] 曹冀为 王琼 汪庭军 XI Tian-yi;YANG Guo-rong;CAO Ji-wei;WANG Qiong;WANG Tin-jun(Department of General Surgery,Suzhou Hospital Affiliated to Nanjing Medical University,Suzhou 215100,China;Hemodialysis Center,Suzhou Hospital Affiliated to Nanjing Medical University,Suzhou 215100,China)
机构地区:[1]南京医科大学附属苏州医院普外科,江苏苏州215100 [2]南京医科大学附属苏州医院血透中心,江苏苏州215100
出 处:《中国现代普通外科进展》2023年第2期85-88,共4页Chinese Journal of Current Advances in General Surgery
摘 要:目的:探讨富含半胱氨酸的酸性分泌蛋白(SPARC)的基因缺失对小鼠脾脏边缘区B细胞抗凋亡机制的影响。方法:10只对SPARC缺失纯合的小鼠指定为缺失组,从对SPARC缺失杂合的小鼠杂交获得。10只野生型(WT)小鼠设置为WT组。小鼠常规饲养到4个月,行小鼠称重和脾脏称重以计算脾脏指数。每组收集5只小鼠脾脏,通过苏木精-伊红染色计算脾脏中白浆的面积百分比。每组5只小鼠脾脏,通过流式细胞术筛选脾边缘区B细胞。结果:缺失组的脾脏质量、脾脏指数、白浆面积百分比和边缘区B细胞百分比均显著高于WT组(P<0.05)。缺失组的体质量低于WT组(P<0.05)。两组在不同时间和相互作用,边缘区B细胞凋亡、Caspase8 mRNA和相对蛋白表达差异均有统计学意义(P<0.05)。缺失组的边缘区B细胞凋亡率、Caspase8 mRNA和蛋白质表达水平均低于WT组(P<0.05)。结论:SPARC基因缺失可引起脾肿大和边缘区B细胞增生,降低脾脏边缘区B细胞凋亡率,可能与SPARC基因缺失导致Caspase8 mRNA和蛋白的异常低表达水平有关。Objective:The aim of this study was to investigate the effect of the deletion of acidic protein and cysteine-rich(SPARC)genes on the anti-apoptotic mechanism of splenic marginal region B cells in mice.Methods:Ten mice with homozygous deletion of SPARC were assigned to the deletion group.Mice were originally hybridized with mice without a SPARC heterozygosity.Ten wild-type(WT)mice were assigned to the WT group.All mice were reared until 4 months of age by conventional methods.Before all the mice were killed,they were weighed and their spleens were weighed to calculate the spleen index.Spleens of five mice in each group were collected and the percentage of white plasma area in the spleens was calculated by hematoxylin-eosin staining.Spleens of five mice in each group were collected and B cells in the marginal region of spleen were screened by flow cytometry.Results:The spleen mass,spleen index,percentage of white plasma area and percentage of marginal B cells in the deletion group were significantly higher than those in the WT group(P<0.05).The body weight of the deletion group was lower than that of the WT group(P<0.05).There were significant differences in the expression of Caspase8 mRNA and relative protein in the apoptosis of B cells in the marginal region between the two groups at different time and interaction(P<0.05).The apoptosis rate and Caspase8 mRNA and protein expression levels of marginal region B cells in the deletion group were significantly lower than those in the WT group(P<0.05).Conclusion:SPARC gene deletion can induce splenomegaly and peripheral B-cell proliferation,and reduce the apoptosis rate of peripheral B-cell in spleen.These phenomena may be related to the abnormal low expression level of Caspase8 mRNA and protein caused by the deletion of SPARC gene.
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