黄芩对ZEA暴露雌性小鼠抗氧化能力、性激素水平和ERαmRNA表达的影响  

Effects of Scutellaria baicalensis georgi on Antioxidant Capacity,Sex Hormone Levels and mRNA Expression of ERαin Female Mice Exposed to ZEA

作　　者：雷奇 任杰[2] 康杰[2] LEI Qi;REN Jie;KANG Jie(DSM Vitamin(Shandong)Co.,Ltd.,Liaocheng 252000,China;College of Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]帝斯曼维生素(山东)有限公司,聊城252000 [2]山西农业大学动物医学学院,太谷030801

出　　处：《中国畜牧兽医》2023年第3期989-997,共9页China Animal Husbandry & Veterinary Medicine

摘　　要：【目的】试验旨在研究黄芩(SBG)对玉米赤霉烯酮(ZEA)暴露幼龄雌性小鼠抗氧化能力、性激素水平及雌性生殖器官中雌激素受体α(ERα)和孕酮受体(PR)基因表达的影响。【方法】试验选取50只雌性小鼠,预饲1周后随机分为5组,每组10只。对照组(CK)灌胃10 mL/kg玉米油、ZEA组灌胃20 mg/kg ZEA、ZEA+SBG10组灌胃20 mg/kg ZEA+10 g/kg SBG、ZEA+SBG20组灌胃20 mg/kg ZEA+20 g/kg SBG、ZEA+SBG30组灌胃20 mg/kg ZEA+30 g/kg SBG。所有试验小鼠均饲喂基础饲粮,正试期3周。正试期间每日记录小鼠体重;分别在试验的第1、2和3周末晨饲前采集小鼠尾静脉血样,检测血清中抗氧化相关指标和性激素含量;在试验第3周末,采集卵巢和子宫,检测相关类固醇激素受体基因mRNA的表达。【结果】与CK组相比,ZEA暴露2周以上可引起雌性小鼠体重、血清谷胱甘肽过氧化物酶(GSH-Px)活性、谷胱甘肽(GSH)和雌二醇(E 2)含量均显著降低(P<0.05);卵巢刺激素(FSH)水平显著增加(P<0.05),而黄体生成素(LH)水平无显著变化(P>0.05)。与ZEA组相比,20和30 g/kg SBG处理3周均显著提升小鼠体重(P<0.05),20 g/kg SBG处理3周显著升高血清总超氧化物歧化酶(T-SOD)、GSH-Px活性及GSH水平(P<0.05),30 g/kg SBG处理2周后这3项指标均显著提高(P<0.05);20和30 g/kg SBG处理2周显著降低血清丙二醛(MDA)含量(P<0.05);30 g/kg SBG处理2周可显著增加血清E 2水平(P<0.05),显著降低FSH含量(P<0.05);20和30 g/kg SBG处理3周后小鼠子宫和卵巢ERα基因表达显著提升(P<0.05),但PR的mRNA表达量在各组之间差异不显著(P>0.05)。【结论】SBG可通过提高ZEA暴露雌性小鼠血清抗氧化能力及性激素水平,增强子宫及卵巢ERα的表达,缓解ZEA造成的氧化损伤和生殖毒性,SBG推荐剂量为20~30 g/kg。【Objective】This experiment was conducted to investigate the effects of Scutellaria baicalensis georgi(SBG)on antioxidant capacity,sex hormone levels and mRNA expression of estrogen receptorsα(Erα)and progesterone receptors(PR)in female mice exposed to zearalenone(ZEA).【Method】Fifty female mice were chosen and randomly divided into five groups after one week pre-feed,with 10 mice in each group.The mice in control group(CK)were given 10 mL/kg corn oil,in ZEA group were given 20 mg/kg ZEA,in ZEA+SBG10 group were given 20 mg/kg ZEA+10 g/kg SBG,in ZEA+SBG20 group were given 20 mg/kg ZEA+20 g/kg SBG,and the mice in ZEA+SBG30 group were given 20 mg/kg ZEA+30 g/kg SBG.All experimental mice were fed a basal diet for 3 weeks.The body weight of mice was recorded daily during the trial period.The blood samples of tail root vein of mice were collected before morning feeding at the end of the first,second and third week,respectively,and the antioxidant-related indexes and sex hormone content in serum were determined.At the end of the third week of the trial,ovaries and uterus were collected to detect the mRNA expression of the relevant steroid hormone receptor gene.【Result】Compared with the CK group,ZEA exposure for more than 2 weeks resulted in a significant decrease in body weight,serum glutathione peroxidase(GSH-Px)activity,glutathione(GSH)and estradiol(E 2)levels(P<0.05),and a significant increase in ovarian stimulating hormone(FSH)levels(P<0.05).While luteinizing hormone(LH)levels had no significant changes(P>0.05).Compared with ZEA group,the body weight of mice treated with 20 or 30 g/kg SBG for 3 weeks was significantly increased(P<0.05),and the activities of total superoxide dismutase(T-SOD)and GSH-Px and GSH level in serum of mice treated with 20 g/kg SBG for 3 weeks were significantly increased(P<0.05).After treatment with 30 g/kg SBG for 2 weeks,these three indexes were significantly increased(P<0.05).Treatment with 20 or 30 g/kg SBG for 2 weeks significantly decreased serum malondialdehyde(MDA)content

关 键 词：黄芩 玉米赤霉烯酮(ZEA) 抗氧化能力 性激素 雌激素受体α(ERα) 孕酮受体(PR) 

分 类 号：S859[农业科学—临床兽医学]