无细胞生物传感器及其在食品与生物检测应用中的研究进展  被引量:1

Research Progress on Cell-Free Biosensor and Its Application in Food and Biological Detection

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作  者:王佳佳 王鑫 庞晓旭 黄昆仑 许文涛 罗云波 程楠 WANG Jiajia;WANG Xin;PANG Xiaoxu;HUANG Kunlun;XU Wentao;LUO Yunbo;CHENG Nan(Beijing Laboratory for Food Quality and Safety,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),Ministry of Agriculture and Rural Affairs,Beijing 100083,China;Beijing Advanced Innovation Center for Food Nutrition and Human Health,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区:[1]中国农业大学食品科学与营养工程学院,食品质量与安全北京实验室,北京100083 [2]农业农村部农业转基因生物安全评价(食用)重点实验室,北京100083 [3]中国农业大学食品科学与营养工程学院,北京食品营养与人类健康高精尖创新中心,北京100083

出  处:《食品科学》2023年第5期355-364,共10页Food Science

基  金:中国农业大学2115人才工程项目。

摘  要:无细胞生物传感器是一类基于无细胞系统设计的微量物质检测工具,解决了早期活细胞生物传感器无法检测有毒物质、响应时间长、受活细胞控制、操作不便等问题。近年来,无细胞生物传感器以其检测范围广、响应速度快、灵敏度高和便携性好而受到广泛关注。本文按照构建无细胞传感器平台进行分类,并对不同类型无细胞传感器的构建和特点进行综述,进一步介绍其在金属离子、农药与兽药残留等领域检测的研究进展,并对其应用前景进行展望。The cell-free biosensor is a tool designed to detect trace substances based on a cell-free system,which solves the problems of the traditional cell-based biosensor including having no ability to detect toxic substances,long response time,being controlled by living cells,and inconvenient operation.In recent years,cell-free biosensors have attracted extensive attention for their wide detection range,fast response speed,high sensitivity and portability.In this paper,the construction and characteristics of different types of cell-free sensors are reviewed.In addition,their applications in the detection of metal ions,pesticide residues and veterinary drug residues are introduced,and future prospects also are discussed.

关 键 词:无细胞生物传感器 检测应用 灵敏度 便携性 液相/纸基生物传感器 

分 类 号:Q819[生物学—生物工程]

 

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