快速革兰氏染色法对大、小鼠动情周期的鉴别  

Identification of estrous cycles in rats and mice by rapid Gram staining

作　　者：张蕾 张若乾 秦秀云 李宏林 张建伟[3] ZHANG Lei;ZHANG Ruoqian;QIN Xiuyun;LI Honglin;ZHANG Jianwei(the First Clinical Medical College of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Department of Traditional Chinese Medicine,Rizhao Maternal and Child Health Care Hospital,Rizhao 276800;Department of Gynecology,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250011)

机构地区：[1]山东中医药大学第一临床医学院,济南250014 [2]日照市妇幼保健院中医科,山东日照276800 [3]山东中医药大学附属医院中西医结合生殖与遗传中心,济南250011

出　　处：《中国比较医学杂志》2023年第3期45-50,74,共7页Chinese Journal of Comparative Medicine

基　　金：山东省自然科学基金面上项目(ZR2020MH363);国家自然科学基金青年科学基金项目(82205174);山东省中医药科技项目(2021M176)。

摘　　要：目的通过快速革兰氏染色法分别对SD雌性大鼠、C57BL/6J雌性小鼠进行阴道涂片染色,镜下观察细胞形态,为快速鉴别动情周期寻求简单易行的染色方法。方法12只SD雌性大鼠和12只C57BL/6J雌性小鼠,56~62日龄,每天9:00采用阴道拭子法获得阴道涂片,干燥后进行快速革兰氏染色法,连续观察12 d,于光镜下观察细胞形态,判断其动情周期阶段。结果快速革兰氏染色法用时较短,操作简单,观察视野色彩鲜明,背景干净,绘制完整的大、小鼠动情周期变化图。结论快速革兰氏染色法可简单、直观的判断大、小鼠动情周期,并优化染色步骤,对染色中出现的异常情况进行逐一分析,将每张涂片染色时间缩短至1 min以内,对实验新手易于掌握,可推广应用。ObjectiveTo subject vaginal smears of female SD rats and C57BL/6J mice to rapid Gram staining,observe the cell morphology,and develop a simple staining method to rapidly identify the estrous cycle stage.Methods Vaginal smears from 12 female SD rats and 12 female C57BL/6J mice at 56~62 days of age were obtained by the vaginal swab method every day at 9:00,dried,and then subjected to rapid Gram staining for continuous observation over 12 days.Cell morphology was observed under a light microscope to determine the estrous cycle stage.ResultsRapid Gram staining was performed quickly and simply,bright in the observation field with a clean background,and provided a complete overview of the changes in the estrous cycle of rats and mice.ConclusionsThe rapid Gram staining method can easily and intuitively judge the estrous cycle stage of rats and mice,staining steps can be optimized,abnormal conditions in the staining can be analyzed stepwise,and the staining time of each smear can be shortened to less than 1 min.The method is easy for novice experimenters to master and can be applied widely.

关 键 词：快速革兰氏染色法 动情周期 阴道涂片 SD大鼠 C57BL/6J小鼠 

分 类 号：R-33[医药卫生]