树鼩眼球组织形态学结构特征与比较分析  

Comparative analysis of the morphological characteristics of tree shrew eyes

作　　者：邱敏 陈柳[1] 霍姝汭 丁相荣 仝品芬[1] 李娜[1] 代解杰[1] 郝佩琪 高家红[1] 陆彩霞[1] QIU Min;CHEN Liu;HUO Shurui;DING Xiangrong;TONG Pinfen;LI Na;DAI Jiejie;HAO Peiqi;GAO Jiahong;LU Caixia(Chinese Academy of Medical Sciences/Peking Union Medical College Institute of Medical Biology,Kunming 650031,China;Kunming Medical University,Kunming 650500)

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,昆明650031 [2]昆明医科大学,昆明650500

出　　处：《中国比较医学杂志》2023年第3期66-74,共9页Chinese Journal of Comparative Medicine

基　　金：云南省科技人才和平台计划项目(2017HC019);云南省重点实验室运行补助专项(2017DG008);国家重点研发计划资助(2021YFF0702404)。

摘　　要：目的获得树鼩眼球组织形态学结构特征,并比较树鼩、恒河猴和人类眼球组织解剖学的异同点,为利用树鼩建立眼部相关疾病模型提供必要的组织学基础数据。方法制作树鼩和恒河猴眼球常规石蜡切片,HE染色后进行分析比较。结果树鼩眼球主要由晶状体和眼球壁组成。眼球壁分3层,由外向内依次为纤维膜、血管膜和视网膜三层。纤维膜前方1/6为角膜,后5/6为巩膜,两者交界处有一移行区域为角巩膜缘;血管膜是眼球壁的中间层,由疏松结缔组织、丰富的血管和色素细胞组成,自前向后分为虹膜、睫状体和脉络膜;视网膜是眼球壁的最内层,呈典型10层结构。树鼩眼球组织学结构组成与恒河猴眼球及人类眼球组成一致,组织各层占比大致相同,不同之处是树鼩巩膜上皮含有黑色素细胞,视网膜各层组织占比与人类不同。结论树鼩眼球组织结构与非人灵长类或人类类似,组织形态大致相同,具备用于建立人类眼科相关疾病动物模型的潜力,以阐明相应眼科疾病致病机制。ObjectiveTo examine the morphological characteristics of the eyeballs of tree shrews and to compare the similarities and differences of the anatomical structure of the eye of tree shrews,monkeys,and humans to provide the necessary histological data to establish ocular disease models using tree shrews.MethodsParaffin-embedded sections of tree shrew and rhesus monkey eyeballs were prepared and analyzed by HE staining.ResultsThe tree shrew eyeball mainly consisted of a lens and eyeball wall.The ocular wall was divided into three layers including the fibrous membrane,vascular membrane,and retina from the outside to inside.The anterior 1/6 of the fibrous membrane was the cornea,and the posterior 5/6 was the sclera.The vascular membrane was the middle layer of the ocular wall.It was composed of loose connective tissue,abundant blood vessels,and pigment cells.It was divided into the iris,ciliary body,and choroid from front to back.The retina was the innermost layer of the eyeball wall and had a typical structure of 10 layers.The histological structure of the tree shrew eyeball was consistent with that of rhesus monkey and human eyeballs,and the proportion of each tissue layer was approximately the same.The main differences were that the sclera epithelium of tree shrews contained melanocytes,and the proportion of each retinal layer was different from that of humans.Conclusions The eye tissue structure of tree shrews is similar to that of non-human primates and humans,and the tissue morphology is approximately the same.There is potential to establish an animal model of human eye-related diseases to clarify the pathogenesis of eye diseases.

关 键 词：树鼩 恒河猴 眼球 组织学结构 

分 类 号：R-33[医药卫生]