桔梗皂苷D增强人直肠癌耐药细胞株SW1463/Oxa对奥沙利铂的敏感性  

Platycodon grandiflorum saponin D enhances the sensitivity of human rectal cancer cell strain SW1463/Oxa with drug resistance to oxaliplatin

作　　者：闫军[1] 冯永安 石永奎 赵志浩 倪怀亮 杨维桢[1] YAN Jun;FENG Yong an;SHI Yongkui;ZHAO Zhihao;NI Huailiang;YANG Weizhen(Department of General Surgery,Baoji Central Hospital,Baoji 721008;Department of Hepatobiliary Pancreatic Splenic Surgery,Baoji Hospital,Xi'an Medical College,Baoji 721006,China)

机构地区：[1]宝鸡市中心医院普外科,陕西宝鸡721008 [2]西安医学院附属宝鸡医院肝胆胰脾外科,陕西宝鸡721006

出　　处：《基础医学与临床》2023年第4期603-607,共5页Basic and Clinical Medicine

基　　金：宝鸡市卫生和计划生育局资助项目(2017-12)。

摘　　要：目的探讨桔梗皂苷D对人直肠癌耐药细胞株SW1463/奥沙利铂(Oxa)化学治疗敏感性的增强作用。方法取人直肠癌细胞系SW1463,建立稳定的耐奥沙利铂细胞株SW1463/Oxa。将构建成功的人直肠癌耐药细胞株SW1463/Oxa随机分为桔梗皂苷D组、Oxa组、桔梗皂苷D+Oxa组和对照组。MTT法检测细胞增殖曲线;RT-qPCR检测脱氧核糖核酸甲基转移酶3a(DNMT3a)、磷酸化组蛋白γ-H2AX、DNA双链修复蛋白RAD51、信号传导子和转录激活子3(STAT3)信使核糖核酸(mRNA)表达;Western blot检测细胞DNMT3a、γ-H2AX、RAD51、STAT3蛋白表达及磷酸化STAT3(p-STAT3)水平。结果成功构建人直肠癌耐药细胞株SW1463/Oxa;与对照组和Oxa组比,桔梗皂苷D组和桔梗皂苷D+Oxa组细胞增殖能力、DNMT3a、RAD51、STAT3 mRNA和蛋白表达以及p-STAT3水平降低(P<0.05),γ-H2AX mRNA和蛋白表达升高(P<0.05)。结论桔梗皂苷D能增强人直肠癌耐药细胞株SW1463/Oxa对Oxa的化疗敏感性,推测是通过抑制DNMT3a、RAD51、STAT3的表达,促进γ-H2AX的表达实现此作用的。Objective To investigate the enhancing effect of platycodon grandiflorum saponin D on thesensitivity of human rectal cancer drug-resistant cell line SW1463 to oxaliplatin(Oxa).Methods Human rectal cancer cell line SW1463 was collected to establish a stable oxaliplatin-resistant cell strain SW1463/Oxa.The constructed cell strain SW1463/Oxa was randomly divided into platycodon grandiflorum saponin D group,Oxa group,platycodonsaponin D+Oxa group and control group.The cell proliferation curve was detected by MTT.Deoxyribonucleic acid methyltransferase 3a(DNMT3a),phosphorylated histoneγ-H2AX,DNA double strand repair protein RAD51 and signal transducer and activator of transcription 3(STAT3)messenger RNA(mRNA)expression were detected by RT-qPCR.DNMT3a,γ-H2AX,RAD51,STAT3 protein expression and phosphorylated STAT3(p-STAT3)level were detected by Western blot.Results Human rectal cancer drug resistant cell strain SW1463/Oxa was successfully constructed.Compared with the control group and Oxa group,the cell proliferation,DNMT3a,RAD51,STAT3 mRNA and protein expression and p-STAT3 level of platycodonsaponin D group and platycodonsaponin D+Oxa group decreased(P<0.05),and the expression ofγ-H2AX mRNA and protein increased(P<0.05).Conclusions Platycodon grandiflorum saponin D may enhance the chemo-sensitivity of human rectal cancer drug-resistant cell strain SW1463/Oxa to Oxa,and the potential mechanism is inhibition of expression of DNMT3a,RAD51,STAT3 and promotion of expression ofγ-H2AX.

关 键 词：桔梗皂苷D 脱氧核糖核酸甲基转移酶3a 直肠癌 敏感性 

分 类 号：R735.3[医药卫生—肿瘤]