环境浓度氧四环素与聚苯乙烯微塑料对黄颡鱼幼鱼肠道的联合毒性效应  

Combined Effects of Environmental Concentration of Oxytetracycline and Polystyrene Microplastics on Intestinal Tract of Juvenile Yellow Catfish(Pelteobagrus fulvidraco)

作　　者：孔娟 范博雅 原居林[2] 余丽琴 Kong Juan;Fan Boya;Yuan Julin;Yu Liqin(College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Zhejiang Institute of Freshwater Fisheries,Huzhou 313000,China;Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan 430070,China)

机构地区：[1]华中农业大学水产学院,武汉430070 [2]浙江省淡水水产研究所,湖州313000 [3]教育部长江经济带大宗水生生物产业绿色发展教育部工程研究中心,武汉430070

出　　处：《生态毒理学报》2023年第1期426-439,共14页Asian Journal of Ecotoxicology

基　　金：农业农村部淡水渔业健康养殖重点实验室开放课题(ZJK202010);中央高校基本科研业务费专项资金(2662021SCPY003)。

摘　　要：近年来,氧四环素(oxytetracycline,OTC)和聚苯乙烯微塑料(polystyrene microplastics,PS-MPs)在水环境中被广泛检出。为了探究PS-MPs与OTC对鱼类的联合毒性效应,选取黄颡鱼(Pelteobagrus fulvidraco)幼鱼为研究对象,将其暴露于CON(对照)组、环境浓度OTC(500 ng·L^(-1))单独组、MPs-L(100μg·L^(-1)PS-MPs)单独组、MPs-L+OTC(100μg·L^(-1)PS-MPs+500 ng·L^(-1)OTC)复合组、MPs-H(1000μg·L^(-1)PS-MPs)单独组和MPs-H+OTC(1000μg·L^(-1)PS-MPs+500 ng·L^(-1)OTC)复合组中28 d,研究了OTC和PS-MPs单独以及联合暴露对幼鱼生长、肠道结构和肠道菌群的影响。研究结果表明,与对照组相比,OTC单独暴露和MPs-L单独暴露对黄颡鱼幼鱼体长、体质量及体质量增长率,肠道氧化应激酶(超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT))活性及消化酶(胰蛋白酶(trypsin,TRS)、淀粉酶(amylase,AMS)和脂肪酶(lipase,LPS))活性,肠道微生物组成(OTU数目、α多样性、β多样性以及门、属水平上物种组成的相对丰度)均无显著性影响。但MPs-L+OTC复合暴露导致SOD和CAT活性显著升高,引起肠道空泡化、肠上皮细胞轻微缺失,变形菌门的相对丰度显著升高,且与OTC单独暴露组相比,肠道CAT活性显著性升高。MPs-H单独暴露抑制了黄颡鱼幼鱼体质量和体质量增长率,引起了肠道空泡化,导致其肠道SOD和CAT活性显著升高,消化酶TRS和LPS活性显著降低,厚壁菌门相对丰度显著降低。与MPs-H单独组和OTC单独组相比,MPs-H+OTC组进一步加剧肠道氧化酶活性升高、消化酶活性降低、肠道损伤和肠道菌群紊乱。相关性分析表明,肠道葡萄球菌属和体长显著负相关;鲸杆菌属和SOD显著正相关;气单胞菌属与LPS显著负相关,与AMS显著正相关。上述结果显示PS-MPs高浓度单独以及与OTC复合暴露可能通过肠道损伤以及肠道菌群的改变,进而影响肠道消化酶活性,导致黄颡鱼幼鱼生长抑制。此外,PS-In recent years,oxytetracycline(OTC)and polystyrene microplastics(PS-MPs)have been widely detected in aquatic environment.In order to investigate the combined effects of PS-MPs and OTC on intestinal tract of fish,we investigated the effects of OTC and PS-MPs exposure on growth,intestinal structure and intestinal microflora of fish.Juvenile yellow catfish(Pelteobagrus fulvidraco)were exposed to 500 ng·L^(-1)OTC(OTC),100(low concentration)(MPs-L)and 1000μg·L^(-1)(high concentration)(MPs-H)PS-MPs,or their combination(combined MPs-L+OTC,combined MPs-H+OTC)for 28 d.Results showed that OTC and MPs-L alone exposure had no significant effect on the growth,intestinal antioxidant enzyme activities(including superoxide dismutase(SOD)and catalase(CAT)),digestive enzyme activities(including trypsin(TRS),amylase(AMS)and lipase(LPS)),or intestinal flora(including OTU number,alpha diversity,beta diversity,and relative abundance of species composition at phylum level and genus level)of juvenile yellow catfish.However,combined MPs-L+OTC exposure significantly increased SOD and CAT activities,induced intestinal vacuolation and slight loss of intestinal epithelial cells,as well as significantly increased the relative abundance of Proteobacteria as compared to the control group.Intestinal CAT activity was significantly increased in the combined MPs-L+OTC exposure group as compared to the OTC alone exposure group.Moreover,MPs-H alone exposure inhibited the body weight and weight gain rate of juvenile yellow catfish,induced intestinal vacuolation,significantly increased the activities of SOD and CAT,significantly decreased the activities of digestive enzymes TRS and LPS,and significantly decreased the relative abundance of Firmicutes.Compared with the MPs-H group and OTC group,the effects on oxidase activities,digestive enzyme activities,intestinal injury and intestinal flora were further exacerbated in the combined MPs-H+OTC group.In addition,correlation analysis showed that a significant negative correlation between Staphylococcus

关 键 词：聚苯乙烯微塑料 氧四环素 黄颡鱼幼鱼 复合暴露 肠道菌群 

分 类 号：X171.5[环境科学与工程—环境科学]